21790

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 253186..253828 Replicon chromosome
Accession NC_008600
Organism Bacillus thuringiensis str. Al Hakam

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BALH_RS01440 Protein ID WP_000635963.1
Coordinates 253478..253828 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BALH_RS01435 Protein ID WP_000004570.1
Coordinates 253186..253473 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BALH_RS01410 248502..249464 + 963 WP_000961158.1 UV DNA damage repair endonuclease UvsE -
BALH_RS01415 249457..250029 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BALH_RS01420 250122..250481 + 360 WP_000635040.1 holo-ACP synthase -
BALH_RS01425 250638..251588 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BALH_RS01430 251707..252876 + 1170 WP_000390600.1 alanine racemase -
BALH_RS01435 253186..253473 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BALH_RS01440 253478..253828 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BALH_RS01445 253896..256064 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BALH_RS01450 256122..256238 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BALH_RS01455 256434..256892 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T21790 WP_000635963.1 NC_008600:253478-253828 [Bacillus thuringiensis str. Al Hakam]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T21790 NC_008600:253478-253828 [Bacillus thuringiensis str. Al Hakam]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT21790 WP_000004570.1 NC_008600:253186-253473 [Bacillus thuringiensis str. Al Hakam]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT21790 NC_008600:253186-253473 [Bacillus thuringiensis str. Al Hakam]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References