Detailed information of TA system TA217898

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3522226..3522905
• Replicon: chromosome
• Accession: NZ_CP083990
• Organism: Escherichia coli strain EC2648-Vir2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: LCH23_RS17150
• Protein ID: WP_000854672.1
• Coordinates: 3522226..3522567 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: LCH23_RS17155
• Protein ID: WP_000070395.1
• Coordinates: 3522588..3522905 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH23_RS17125 (3517812)	3517812..3517904	+	93	Protein_3367	sigma factor-binding protein Crl	-
LCH23_RS17130 (3517943)	3517943..3518998	-	1056	WP_000749863.1	phosphoporin PhoE	-
LCH23_RS17135 (3519286)	3519286..3520389	+	1104	WP_001285288.1	glutamate 5-kinase	-
LCH23_RS17140 (3520401)	3520401..3521654	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
LCH23_RS17150 (3522226)	3522226..3522567	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
LCH23_RS17155 (3522588)	3522588..3522905	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LCH23_RS17160 (3522924)	3522924..3523145	-	222	WP_000691994.1	DUF987 domain-containing protein	-
LCH23_RS17165 (3523154)	3523154..3523630	-	477	WP_000811693.1	RadC family protein	-
LCH23_RS17170 (3523646)	3523646..3524104	-	459	WP_000211838.1	antirestriction protein	-
LCH23_RS17175 (3524202)	3524202..3524441	-	240	WP_000194654.1	DUF905 family protein	-
LCH23_RS17180 (3524518)	3524518..3524985	-	468	WP_001547765.1	protein YkfB	-
LCH23_RS17185 (3525008)	3525008..3525451	-	444	WP_000824223.1	lipoprotein YafY	-
LCH23_RS17190 (3525451)	3525451..3525678	-	228	WP_001548158.1	protein YpjK	-
LCH23_RS17195 (3525674)	3525674..3525865	-	192	Protein_3380	DeoR family transcriptional regulator	-
LCH23_RS17200 (3526082)	3526082..3526903	-	822	WP_000197389.1	DUF932 domain-containing protein	-
LCH23_RS17205 (3526995)	3526995..3527858	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3517106..3555919	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T217898 WP_000854672.1 NZ_CP083990:c3522567-3522226 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T217898 NZ_CP114893:3524646-3525968 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT217898 WP_000070395.1 NZ_CP083990:c3522905-3522588 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT217898 NZ_CP114893:3524380-3524646 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684