217896

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2369571..2370209 Replicon chromosome
Accession NZ_CP083990
Organism Escherichia coli strain EC2648-Vir2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LCH23_RS11470 Protein ID WP_000813794.1
Coordinates 2370033..2370209 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LCH23_RS11465 Protein ID WP_001270286.1
Coordinates 2369571..2369987 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH23_RS11445 (2364723) 2364723..2365664 - 942 WP_001251304.1 ABC transporter permease -
LCH23_RS11450 (2365665) 2365665..2366678 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
LCH23_RS11455 (2366696) 2366696..2367841 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LCH23_RS11460 (2368086) 2368086..2369492 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LCH23_RS11465 (2369571) 2369571..2369987 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LCH23_RS11470 (2370033) 2370033..2370209 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LCH23_RS11475 (2370431) 2370431..2370661 + 231 WP_000494244.1 YncJ family protein -
LCH23_RS11480 (2370753) 2370753..2372714 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LCH23_RS11485 (2372787) 2372787..2373323 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LCH23_RS11490 (2373415) 2373415..2374590 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T217896 WP_000813794.1 NZ_CP083990:c2370209-2370033 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T217896 NZ_CP114893:3192668-3192771 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT217896 WP_001270286.1 NZ_CP083990:c2369987-2369571 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT217896 NZ_CP114893:c3192809-3192538 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References