Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1802328..1803159 Replicon chromosome
Accession NZ_CP083990
Organism Escherichia coli strain EC2648-Vir2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LCH23_RS08580 Protein ID WP_000854814.1
Coordinates 1802328..1802702 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag LCH23_RS08585 Protein ID WP_001285584.1
Coordinates 1802791..1803159 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH23_RS08540 (1797724) 1797724..1798890 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
LCH23_RS08545 (1799009) 1799009..1799482 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
LCH23_RS08550 (1799680) 1799680..1800738 + 1059 WP_001200891.1 FUSC family protein -
LCH23_RS08555 (1800910) 1800910..1801239 + 330 WP_000450409.1 DUF496 family protein -
LCH23_RS08560 (1801340) 1801340..1801474 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
LCH23_RS08565 (1801594) 1801594..1801722 + 129 Protein_1682 transposase domain-containing protein -
LCH23_RS08570 (1802011) 1802011..1802091 - 81 Protein_1683 hypothetical protein -
LCH23_RS08575 (1802137) 1802137..1802331 - 195 WP_000988600.1 DUF5983 family protein -
LCH23_RS08580 (1802328) 1802328..1802702 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LCH23_RS08585 (1802791) 1802791..1803159 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LCH23_RS08590 (1803233) 1803233..1803454 - 222 WP_000692323.1 DUF987 domain-containing protein -
LCH23_RS08595 (1803517) 1803517..1803963 - 447 WP_000187523.1 RadC family protein -
LCH23_RS08600 (1803960) 1803960..1805492 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T217885 WP_000854814.1 NZ_CP083990:c1802702-1802328 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T217885 NZ_CP114893:1404770-1404877 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT217885 WP_001285584.1 NZ_CP083990:c1803159-1802791 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT217885 NZ_CP114893:c1404722-1404656 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References