Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4528883..4529424 Replicon chromosome
Accession NC_008596
Organism Mycobacterium smegmatis str. MC2 155

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag MSMEG_RS21490 Protein ID WP_003895797.1
Coordinates 4529095..4529424 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag MSMEG_RS21485 Protein ID WP_011729843.1
Coordinates 4528883..4529092 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSMEG_RS21460 (MSMEG_4440) 4524154..4524924 - 771 WP_011729837.1 SDR family oxidoreductase -
MSMEG_RS21465 (MSMEG_4441) 4524954..4525862 - 909 WP_011729838.1 cupin domain-containing protein -
MSMEG_RS21470 (MSMEG_4442) 4525892..4526812 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
MSMEG_RS21475 (MSMEG_4443) 4526902..4527093 - 192 Protein_4261 hypothetical protein -
MSMEG_RS21480 (MSMEG_4446) 4527310..4528722 + 1413 Protein_4262 dihydrolipoyl dehydrogenase -
MSMEG_RS21485 (MSMEG_4447) 4528883..4529092 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
MSMEG_RS21490 (MSMEG_4448) 4529095..4529424 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MSMEG_RS21495 (MSMEG_4449) 4529467..4530030 - 564 WP_014878038.1 TetR/AcrR family transcriptional regulator -
MSMEG_RS21500 (MSMEG_4450) 4530136..4531071 + 936 WP_003895799.1 alpha/beta fold hydrolase -
MSMEG_RS21505 (MSMEG_4451) 4531068..4532780 + 1713 WP_011729844.1 NAD(P)/FAD-dependent oxidoreductase -
MSMEG_RS21515 (MSMEG_4453) 4532997..4533377 + 381 WP_029104585.1 phage holin family protein -
MSMEG_RS21520 (MSMEG_4454) 4533395..4534420 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T21788 WP_003895797.1 NC_008596:4529095-4529424 [Mycolicibacterium smegmatis MC2 155]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T21788 NC_008596:4529095-4529424 [Mycolicibacterium smegmatis MC2 155]
GTGCGGCGCGGCGATATCTACACCGCGGCGGCGCGAGGTGCGTACACCGGCAAGCCTCGGCCGGTCCTCATCATCCAGGA
CGACCGATTCGATGCCACCGCGTCGGTCACCGTCGTGCCGTTTACGACAAGCGACACCGACGCACCGCTTCTGCGAATTC
GAATCGAGCCAACGCCAGCCACCGGACTCTCCACGGTCAGCTCGTTGATGATCGACAAGGTGACCACAGTTCCCCGCTCC
AGCTTGACGCACCGTGTCGGCCGACTGGCGGAGACAGACATGGTCAGGACCGACCGCGCACTCCTGGTTTTTCTGGGAAT
CGCCGGGTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT21788 WP_011729843.1 NC_008596:4528883-4529092 [Mycolicibacterium smegmatis MC2 155]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT21788 NC_008596:4528883-4529092 [Mycolicibacterium smegmatis MC2 155]
ATGACTCCCGCGCGTGACCGAGTACGCCGACATCGCGAGCGGCTGCGGCGACAAGGTCTGCGGCCGGTGCAGATCTGGGT
CCCCGACGTGAATGCACCCGAATTTCGCCGGGAGGCGCACCGGCAGTCTGAGTTGGTCGCCGCCGGCGAGCATGAGGCCG
AAGATCAAGCTTTCGTCGACGCGATCTCCGTCGACTGGGACGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References