Detailed information of TA system TA217874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 478902..479464
• Replicon: plasmid pGD0221
• Accession: NZ_CP083975
• Organism: Rhodococcus rhodochrous strain GD02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KUM34_RS27750
• Protein ID: WP_085470959.1
• Coordinates: 478902..479192 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KUM34_RS27755
• Protein ID: WP_085470958.1
• Coordinates: 479189..479464 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUM34_RS27730 (KUM34_027735)	474801..476031	-	1231	Protein_430	IS3 family transposase	-
KUM34_RS27735 (KUM34_027740)	476160..476393	+	234	WP_179161884.1	hypothetical protein	-
KUM34_RS27740 (KUM34_027745)	476483..477053	-	571	Protein_432	SDR family NAD(P)-dependent oxidoreductase	-
KUM34_RS27750 (KUM34_027755)	478902..479192	-	291	WP_085470959.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUM34_RS27755 (KUM34_027760)	479189..479464	-	276	WP_085470958.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KUM34_RS27760 (KUM34_027765)	479937..480110	+	174	WP_230825810.1	hypothetical protein	-
KUM34_RS27765	480148..480282	+	135	WP_256062113.1	hypothetical protein	-
KUM34_RS27770 (KUM34_027770)	480732..481256	+	525	WP_229583149.1	hypothetical protein	-
KUM34_RS27775 (KUM34_027775)	481253..481873	+	621	WP_229583151.1	RES family NAD+ phosphorylase	-
KUM34_RS27780 (KUM34_027780)	482021..482257	+	237	Protein_440	NADP-dependent isocitrate dehydrogenase	-
KUM34_RS27785 (KUM34_027785)	482895..484352	-	1458	WP_265572788.1	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..721409	721409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (14-94)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10818.32 Da
• Isoelectric Point: 10.1476

>T217874 WP_085470959.1 NZ_CP083975:c479192-478902 [Rhodococcus rhodochrous]
VSDNPAARPGPYHVEVASPARRDLGRLPPRIVHAVIEFISGPLADNPHRLSKPLRDDLEGLHSARRGDYRILLRIDDPNH
TIVIVKIDHRAHAYRT

Nucleotide

• Length: 291 bp

>T217874 NZ_CP114892:c4042039-4041761 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9749.91 Da
• Isoelectric Point: 4.4936

>AT217874 WP_085470958.1 NZ_CP083975:c479464-479189 [Rhodococcus rhodochrous]
MTTVPLGEAKDKLSALVDSAETTHDIITITKHGKPAAVLMSADDLESLHETIYWLSRADTRDAVAAADAEYAAGQTISLE
ELRAEHGMPSK

Nucleotide

• Length: 276 bp

>AT217874 NZ_CP114892:c4042302-4042042 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA