Detailed information of TA system TA217873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 400893..401461
• Replicon: plasmid pGD0221
• Accession: NZ_CP083975
• Organism: Rhodococcus rhodochrous strain GD02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KUM34_RS27390
• Protein ID: WP_229583104.1
• Coordinates: 401105..401461 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KUM34_RS27385
• Protein ID: WP_145709042.1
• Coordinates: 400893..401108 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUM34_RS27365 (KUM34_027370)	396003..396527	-	525	WP_120285125.1	nucleotide exchange factor GrpE	-
KUM34_RS27370 (KUM34_027375)	396536..398416	-	1881	WP_229583106.1	molecular chaperone DnaK	-
KUM34_RS27375 (KUM34_027380)	399823..399959	+	137	Protein_359	IS30 family transposase	-
KUM34_RS27380 (KUM34_027385)	400161..400517	+	357	WP_229583212.1	hypothetical protein	-
KUM34_RS27385 (KUM34_027390)	400893..401108	+	216	WP_145709042.1	ribbon-helix-helix domain-containing protein	Antitoxin
KUM34_RS27390 (KUM34_027395)	401105..401461	+	357	WP_229583104.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KUM34_RS27395 (KUM34_027400)	402024..402563	+	540	WP_229583101.1	Abi family protein	-
KUM34_RS27400 (KUM34_027405)	402883..403218	+	336	WP_120285133.1	GNAT family N-acetyltransferase	-
KUM34_RS27405 (KUM34_027410)	403215..403694	+	480	WP_229581069.1	carboxymuconolactone decarboxylase family protein	-
KUM34_RS27410 (KUM34_027415)	403761..403874	+	114	Protein_366	NYN domain-containing protein	-
KUM34_RS27415 (KUM34_027420)	403880..404659	-	780	WP_229581068.1	ABC transporter ATP-binding protein	-
KUM34_RS27420 (KUM34_027425)	404656..405771	-	1116	WP_229583099.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..721409	721409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-117)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-41)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12982.75 Da
• Isoelectric Point: 10.4220

>T217873 WP_229583104.1 NZ_CP083975:401105-401461 [Rhodococcus rhodochrous]
MIRRGHVHWVDLDPAQGSEAAKRRPVVVVSNDRANATAERLGRGVVTVVPLTNNTAHVFAFQVLVEPDESGMPRESKAQA
EQVRSVSVRRLDSDPVGKLSTRTLAALDEALRLHLDLR

Nucleotide

• Length: 357 bp

>T217873 NZ_CP114892:c3768105-3767848 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7790.50 Da
• Isoelectric Point: 4.1111

>AT217873 WP_145709042.1 NZ_CP083975:400893-401108 [Rhodococcus rhodochrous]
MTTRFTISLPDEDARVLDAYVRDHGLGGRSAGIRAALRMLREVGLERDYATAFVEGGAEDDDWDVTSADSE

Nucleotide

• Length: 216 bp

>AT217873 NZ_CP114892:c3767836-3767291 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA