Detailed information of TA system TA217872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3068341..3069293
• Replicon: chromosome
• Accession: NZ_CP083974
• Organism: Rhodococcus rhodochrous strain GD02

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: KUM34_RS14100
• Protein ID: WP_229582042.1
• Coordinates: 3068341..3068910 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A385LF66
• Locus tag: KUM34_RS14105
• Protein ID: WP_039585981.1
• Coordinates: 3068907..3069293 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUM34_RS14080 (KUM34_014070)	3063540..3064655	+	1116	WP_229579265.1	FAD-dependent monooxygenase	-
KUM34_RS14085 (KUM34_014075)	3064695..3065621	+	927	WP_229582039.1	alpha/beta hydrolase	-
KUM34_RS14090 (KUM34_014080)	3065618..3066559	+	942	WP_229582040.1	MBL fold metallo-hydrolase	-
KUM34_RS14095 (KUM34_014085)	3066580..3068310	+	1731	WP_229582041.1	CocE/NonD family hydrolase	-
KUM34_RS14100 (KUM34_014090)	3068341..3068910	-	570	WP_229582042.1	zeta toxin family protein	Toxin
KUM34_RS14105 (KUM34_014095)	3068907..3069293	-	387	WP_039585981.1	hypothetical protein	Antitoxin
KUM34_RS14110 (KUM34_014100)	3069500..3070570	+	1071	WP_229582043.1	class I SAM-dependent methyltransferase	-
KUM34_RS14115 (KUM34_014105)	3070638..3071003	+	366	WP_085470260.1	HNH endonuclease	-
KUM34_RS14120 (KUM34_014110)	3071059..3071223	-	165	WP_229582044.1	hypothetical protein	-
KUM34_RS14125 (KUM34_014115)	3072389..3073321	-	933	WP_059384162.1	TAXI family TRAP transporter solute-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-79)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 20439.54 Da
• Isoelectric Point: 10.3998

>T217872 WP_229582042.1 NZ_CP083974:c3068910-3068341 [Rhodococcus rhodochrous]
MRRLDLIVGPNGSGKSTFVELSLAPLLPGSVFVNADVIARQRWPDRAEEMSYEAAQLAASARDRLLRQGRSFIAETVFSH
PSKLELIDRAHEAGFTVVLHVMLVPEAMSVLRVQHRVASGGHAVPEDKIRARYRRLWPLVVEAIGRADQASVYDTASGTP
VVVARFAGGVPVGAVRWPGWAPAALTGLG

Nucleotide

• Length: 570 bp

>T217872 NZ_CP114892:3681298-3681648 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14052.52 Da
• Isoelectric Point: 4.7096

>AT217872 WP_039585981.1 NZ_CP083974:c3069293-3068907 [Rhodococcus rhodochrous]
MTSSTDKVTRFDAELVDSAIAEGGRQNRTGRQQLEYWARIGRAMTAHETASLHRVHEALAGTRELSELTAAEGRLFDAEI
DARLADGLARTDYAEILAARGVTTVVLDDEGRLVEQRPDGSRRVLDDA

Nucleotide

• Length: 387 bp

>AT217872 NZ_CP114892:3681053-3681304 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A385LF66