Detailed information of TA system TA217868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 4298623..4299217
• Replicon: chromosome
• Accession: NZ_CP083956
• Organism: Mesorhizobium sp. B1-1-8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FJ974_RS20935
• Protein ID: WP_140536747.1
• Coordinates: 4298623..4298811 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FJ974_RS20940
• Protein ID: WP_140536750.1
• Coordinates: 4298816..4299217 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ974_RS20910 (FJ974_20910)	4294137..4294736	-	600	WP_140536733.1	hypothetical protein	-
FJ974_RS20915 (FJ974_20915)	4294916..4295509	-	594	WP_264296774.1	outer membrane beta-barrel protein	-
FJ974_RS20920 (FJ974_20920)	4295825..4296814	-	990	WP_140536738.1	Coat protein	-
FJ974_RS20925 (FJ974_20925)	4296860..4297765	-	906	WP_140536741.1	hypothetical protein	-
FJ974_RS20930 (FJ974_20930)	4297983..4298555	-	573	WP_140536744.1	hypothetical protein	-
FJ974_RS20935 (FJ974_20935)	4298623..4298811	+	189	WP_140536747.1	type II toxin-antitoxin system HicA family toxin	Toxin
FJ974_RS20940 (FJ974_20940)	4298816..4299217	+	402	WP_140536750.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FJ974_RS20945 (FJ974_20945)	4299419..4299736	+	318	WP_140536753.1	YMGG-like glycine zipper-containing protein	-
FJ974_RS20950 (FJ974_20950)	4299784..4300947	-	1164	WP_140539332.1	IS4 family transposase	-
FJ974_RS20955 (FJ974_20955)	4301250..4301708	-	459	WP_140539273.1	hypothetical protein	-
FJ974_RS20960 (FJ974_20960)	4302288..4302908	-	621	WP_140539274.1	DUF1851 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4280440..4322166	41726
	-	flank	IS/Tn	-	-	4299784..4300947	1163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6975.14 Da
• Isoelectric Point: 11.4427

>T217868 WP_140536747.1 NZ_CP083956:4298623-4298811 [Mesorhizobium sp. B1-1-8]
MKSADVIDSLLADGWFEVGRKGSHAQFKHRRKPGRVTVPHPKRDIPIGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T217868 NZ_CP114892:c2464443-2464129 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14262.96 Da
• Isoelectric Point: 4.3078

>AT217868 WP_140536750.1 NZ_CP083956:4298816-4299217 [Mesorhizobium sp. B1-1-8]
MRHYIGLIHKEADSDFGVSFPDFPGVVTAGTTLDDARAMAEEALAFHVDGLVEDGEAIPEPSSLDDVMADSDNRMGVAIL
VALKAEAAKVVRVNVSIPEDVLSEIDRYAERHGYSRSGFLTAAAKQAMRNEAA

Nucleotide

• Length: 402 bp

>AT217868 NZ_CP114892:c2464132-2463716 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA