Detailed information of TA system TA217865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3918003..3918614
• Replicon: chromosome
• Accession: NZ_CP083956
• Organism: Mesorhizobium sp. B1-1-8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FJ974_RS19060
• Protein ID: WP_140538361.1
• Coordinates: 3918003..3918197 (+)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FJ974_RS19065
• Protein ID: WP_140538362.1
• Coordinates: 3918216..3918614 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ974_RS19030 (FJ974_19030)	3913402..3913746	-	345	WP_140538355.1	cupin domain-containing protein	-
FJ974_RS19035 (FJ974_19035)	3913753..3915033	-	1281	WP_140538356.1	O-acetylhomoserine aminocarboxypropyltransferase	-
FJ974_RS19040 (FJ974_19040)	3915194..3915715	-	522	WP_140538357.1	CoA-binding protein	-
FJ974_RS19045 (FJ974_19045)	3915712..3916488	-	777	WP_140538358.1	AAA family ATPase	-
FJ974_RS19050 (FJ974_19050)	3916534..3917355	-	822	WP_140538359.1	enoyl-CoA hydratase	-
FJ974_RS19055 (FJ974_19055)	3917499..3917960	+	462	WP_140538360.1	PaaI family thioesterase	-
FJ974_RS19060 (FJ974_19060)	3918003..3918197	+	195	WP_140538361.1	type II toxin-antitoxin system HicA family toxin	Toxin
FJ974_RS19065 (FJ974_19065)	3918216..3918614	+	399	WP_140538362.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FJ974_RS19070 (FJ974_19070)	3918892..3919356	+	465	WP_140538363.1	50S ribosomal protein L13	-
FJ974_RS19075 (FJ974_19075)	3919356..3919832	+	477	WP_140538364.1	30S ribosomal protein S9	-
FJ974_RS19080 (FJ974_19080)	3919890..3920798	-	909	WP_140538365.1	LysR family transcriptional regulator	-
FJ974_RS19085 (FJ974_19085)	3920894..3921766	+	873	WP_140538366.1	aldo/keto reductase family oxidoreductase	-
FJ974_RS19090 (FJ974_19090)	3921939..3922997	-	1059	WP_140538367.1	glycerophosphodiester phosphodiesterase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (7-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7218.41 Da
• Isoelectric Point: 11.1166

>T217865 WP_140538361.1 NZ_CP083956:3918003-3918197 [Mesorhizobium sp. B1-1-8]
MLERDSRKIVKRLKDDGFELVSVRGSHHKFRKGEITLIVPHPEKDLPTGTARAIAKQAGWIRAG

Nucleotide

• Length: 195 bp

>T217865 NZ_CP114892:c2135876-2135541 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14283.14 Da
• Isoelectric Point: 4.6195

>AT217865 WP_140538362.1 NZ_CP083956:3918216-3918614 [Mesorhizobium sp. B1-1-8]
MKNYFALVDKDADSAFGIRFPDIPGCFSAADDAKDIVPNATEALQLWAEDMPLPEPSSHEAVIALPDIRRALADGSYLVS
VPLIDNDSAVVRANVTFERGVLRAIDAAARERGITRSAFLSSAARKEIEAKH

Nucleotide

• Length: 399 bp

>AT217865 NZ_CP114892:c2136124-2135876 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA