Detailed information of TA system TA217863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-AbkA/BrnT-BrnA
• Location: 982987..983497
• Replicon: chromosome
• Accession: NZ_CP083956
• Organism: Mesorhizobium sp. B1-1-8

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FJ974_RS04790
• Protein ID: WP_264296815.1
• Coordinates: 983258..983497 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: AbkA
• Uniprot ID: -
• Locus tag: FJ974_RS04785
• Protein ID: WP_140538120.1
• Coordinates: 982987..983277 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ974_RS04760 (FJ974_04760)	978990..979967	+	978	WP_181177224.1	ABC transporter permease	-
FJ974_RS04765 (FJ974_04765)	979971..980918	+	948	WP_140536457.1	ABC transporter permease	-
FJ974_RS04770 (FJ974_04770)	981075..982025	-	951	WP_226891475.1	IS481 family transposase	-
FJ974_RS04775 (FJ974_04775)	982139..982507	+	369	WP_140538140.1	VOC family protein	-
FJ974_RS04780 (FJ974_04780)	982524..982982	+	459	WP_140538122.1	SRPBCC family protein	-
FJ974_RS04785 (FJ974_04785)	982987..983277	-	291	WP_140538120.1	BrnA antitoxin family protein	Antitoxin
FJ974_RS04790 (FJ974_04790)	983258..983497	-	240	WP_264296815.1	BrnT family toxin	Toxin
FJ974_RS04795 (FJ974_04795)	983599..984366	-	768	WP_140538119.1	TerC family protein	-
FJ974_RS04800 (FJ974_04800)	984447..985271	-	825	WP_140538117.1	YmdB family metallophosphoesterase	-
FJ974_RS04805 (FJ974_04805)	985268..985618	-	351	WP_226891478.1	transcriptional regulator	-
FJ974_RS04815 (FJ974_04815)	985908..986492	-	585	WP_140538116.1	5-formyltetrahydrofolate cyclo-ligase	-
FJ974_RS04825 (FJ974_04825)	987011..987109	+	99	Protein_957	ATP-binding protein	-
FJ974_RS04830 (FJ974_04830)	987319..987765	+	447	WP_140538114.1	GNAT family N-acetyltransferase	-
FJ974_RS04835 (FJ974_04835)	988009..988413	+	405	WP_140538113.1	peptide-methionine (R)-S-oxide reductase MsrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	981075..982025	950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-67)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (29-88)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 8921.23 Da
• Isoelectric Point: 5.5812

>T217863 WP_264296815.1 NZ_CP083956:c983497-983258 [Mesorhizobium sp. B1-1-8]
MGVAFDLAQKVFDEPLHLIVPDRFEDGEQRWHAIGMVGTQVILLVVHTYPTVADEERVRIVGARKAAPYGRKCYEQEGP

Nucleotide

• Length: 240 bp

>T217863 NZ_CP114892:1984809-1985882 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11453.78 Da
• Isoelectric Point: 6.4757

>AT217863 WP_140538120.1 NZ_CP083956:c983277-982987 [Mesorhizobium sp. B1-1-8]
MSRKDLNQDQRDQLAKLTDLPDDQIDTLDIPEAPAENWAYARRGEFYRPLKQPVTIRLDADVLDWFREHVKKGGGYQTEI
NRVLRHYVTEQERRRS

Nucleotide

• Length: 291 bp

>AT217863 NZ_CP114892:1985875-1986246 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA