Detailed information of TA system TA217862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 351726..352278
• Replicon: chromosome
• Accession: NZ_CP083956
• Organism: Mesorhizobium sp. B1-1-8

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FJ974_RS01610
• Protein ID: WP_140531499.1
• Coordinates: 352024..352278 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FJ974_RS01605
• Protein ID: WP_140531501.1
• Coordinates: 351726..352037 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ974_RS01580 (FJ974_01580)	347662..348036	-	375	WP_140531515.1	hydroxyisourate hydrolase	-
FJ974_RS01585 (FJ974_01585)	348232..349656	+	1425	WP_140531512.1	allantoinase PuuE	-
FJ974_RS01590 (FJ974_01590)	349757..350617	+	861	WP_140531509.1	bifunctional allantoicase/(S)-ureidoglycine aminohydrolase	-
FJ974_RS01595 (FJ974_01595)	350614..351105	+	492	WP_140531507.1	ureidoglycolate lyase	-
FJ974_RS01600 (FJ974_01600)	351122..351715	+	594	WP_140531504.1	nucleoside deaminase	-
FJ974_RS01605 (FJ974_01605)	351726..352037	-	312	WP_140531501.1	BrnA antitoxin family protein	Antitoxin
FJ974_RS01610 (FJ974_01610)	352024..352278	-	255	WP_140531499.1	BrnT family toxin	Toxin
FJ974_RS01615 (FJ974_01615)	352461..354446	+	1986	WP_140531496.1	CocE/NonD family hydrolase	-
FJ974_RS01620 (FJ974_01620)	354595..356181	+	1587	WP_140531493.1	ABC transporter substrate-binding protein	-
FJ974_RS01625 (FJ974_01625)	356198..357157	+	960	WP_140531490.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (31-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9462.00 Da
• Isoelectric Point: 8.6380

>T217862 WP_140531499.1 NZ_CP083956:c352278-352024 [Mesorhizobium sp. B1-1-8]
MKIVWDEPKRVTNLENRGLDFADLDIEFFATSIVLPAKAGRLKAVGEFGEVILAVIFKPLGSEAISVISMRHASRKERSV
YEQR

Nucleotide

• Length: 255 bp

>T217862 NZ_CP114892:c1307849-1307598 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11655.18 Da
• Isoelectric Point: 10.2395

>AT217862 WP_140531501.1 NZ_CP083956:c352037-351726 [Mesorhizobium sp. B1-1-8]
MSNAKSHIRRLTDAEEAEIQRQIAADPEDGDATDEQLTQAKSFAEALPELFESIRRSRGRPALEKPKQVVSIRLDQDVVR
KFKATGKGWQARINEVLKNAKVR

Nucleotide

• Length: 312 bp

>AT217862 NZ_CP114892:c1307601-1307347 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA