Detailed information of TA system TA217849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 1207845..1208500
• Replicon: chromosome
• Accession: NZ_CP083952
• Organism: Mesorhizobium sp. B2-1-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FJ970_RS05845
• Protein ID: WP_140754940.1
• Coordinates: 1207845..1208246 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: X6F8Y5
• Locus tag: FJ970_RS05850
• Protein ID: WP_023771771.1
• Coordinates: 1208246..1208500 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ970_RS05825 (FJ970_05825)	1203303..1204337	-	1035	WP_140754948.1	aldo/keto reductase	-
FJ970_RS05830 (FJ970_05830)	1204393..1205574	-	1182	WP_140754946.1	MFS transporter	-
FJ970_RS05835 (FJ970_05835)	1205697..1206602	+	906	WP_140754944.1	LysR family transcriptional regulator	-
FJ970_RS05840 (FJ970_05840)	1206664..1207713	-	1050	WP_140754942.1	aldo/keto reductase	-
FJ970_RS05845 (FJ970_05845)	1207845..1208246	-	402	WP_140754940.1	type II toxin-antitoxin system VapC family toxin	Toxin
FJ970_RS05850 (FJ970_05850)	1208246..1208500	-	255	WP_023771771.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FJ970_RS05855 (FJ970_05855)	1208574..1209236	-	663	WP_140754938.1	YggS family pyridoxal phosphate-dependent enzyme	-
FJ970_RS05860 (FJ970_05860)	1209391..1212015	+	2625	WP_140754936.1	leucine--tRNA ligase	-
FJ970_RS05865 (FJ970_05865)	1212002..1212580	+	579	WP_140754934.1	LPS assembly lipoprotein LptE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14919.98 Da
• Isoelectric Point: 4.7569

>T217849 WP_140754940.1 NZ_CP083952:c1208246-1207845 [Mesorhizobium sp. B2-1-8]
MYLLDTNIVSDVQRRLHRPTEWLASVDPTSVNLSVITLGEIERGIVKLGKVDSEKATRLDLWLRELRRDNADRILAVTED
VALTWDRITAGRTRGSADTLIAATALVHDLILVTRNVTDFEDTGVTVLNPWDV

Nucleotide

• Length: 402 bp

>T217849 NZ_CP114891:c3487163-3486813 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9631.83 Da
• Isoelectric Point: 5.8506

>AT217849 WP_023771771.1 NZ_CP083952:c1208500-1208246 [Mesorhizobium sp. B2-1-8]
MNWHLQDAKNNFSKLVQRARSEGPQTVTLRGERAAVVLSAEDYDRLAGKKKSLAEYLLTGPVWDDDFAEEVNRRPETMIR
NVDL

Nucleotide

• Length: 255 bp

>AT217849 NZ_CP114891:c3487408-3487157 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: X6F8Y5