Detailed information of TA system TA217844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 396739..397291
• Replicon: chromosome
• Accession: NZ_CP083951
• Organism: Mesorhizobium sp. B2-8-5

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FJ430_RS01870
• Protein ID: WP_140702231.1
• Coordinates: 397037..397291 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FJ430_RS01865
• Protein ID: WP_140702233.1
• Coordinates: 396739..397050 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ430_RS01840 (FJ430_01840)	392782..393156	-	375	WP_140702243.1	hydroxyisourate hydrolase	-
FJ430_RS01845 (FJ430_01845)	393325..394749	+	1425	WP_140702241.1	allantoinase PuuE	-
FJ430_RS01850 (FJ430_01850)	394771..395631	+	861	WP_140702239.1	bifunctional allantoicase/(S)-ureidoglycine aminohydrolase	-
FJ430_RS01855 (FJ430_01855)	395628..396119	+	492	WP_140702237.1	ureidoglycolate lyase	-
FJ430_RS01860 (FJ430_01860)	396135..396728	+	594	WP_140702235.1	nucleoside deaminase	-
FJ430_RS01865 (FJ430_01865)	396739..397050	-	312	WP_140702233.1	BrnA antitoxin family protein	Antitoxin
FJ430_RS01870 (FJ430_01870)	397037..397291	-	255	WP_140702231.1	BrnT family toxin	Toxin
FJ430_RS01875 (FJ430_01875)	397477..399462	+	1986	WP_140702229.1	CocE/NonD family hydrolase	-
FJ430_RS01880 (FJ430_01880)	399610..401199	+	1590	WP_140702227.1	ABC transporter substrate-binding protein	-
FJ430_RS01885 (FJ430_01885)	401300..402259	+	960	WP_140702225.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (31-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9505.07 Da
• Isoelectric Point: 8.5759

>T217844 WP_140702231.1 NZ_CP083951:c397291-397037 [Mesorhizobium sp. B2-8-5]
MKIVWDEPKRVTNLANRGLDFSDLDIEFFATSIVLPAKAGRLKAIGEFGEIILAVIFKPLGYEAISVISMRHASRKERSV
YEQH

Nucleotide

• Length: 255 bp

>T217844 NZ_CP114891:2927900-2928118 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11709.31 Da
• Isoelectric Point: 10.4766

>AT217844 WP_140702233.1 NZ_CP083951:c397050-396739 [Mesorhizobium sp. B2-8-5]
MSNTKSHIRRLTDAEEAEIQRQIAADPEDGEATDAQLTQAKPFAEVLPELFESIRRSRGRPTLEKPKQVVSIRLDQDVVR
KFKATGKGWQARINEVLKNAKVR

Nucleotide

• Length: 312 bp

>AT217844 NZ_CP114891:2927500-2927874 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG