Detailed information of TA system TA217838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 285..869
• Replicon: plasmid unnamed2
• Accession: NZ_CP083949
• Organism: Acinetobacter johnsonii strain CSUSB1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: LCH18_RS17695
• Protein ID: WP_000286964.1
• Coordinates: 285..587 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LCH18_RS17700
• Protein ID: WP_000985610.1
• Coordinates: 588..869 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH18_RS17695 (LCH18_17695)	285..587	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCH18_RS17700 (LCH18_17700)	588..869	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
LCH18_RS17705 (LCH18_17705)	956..1498	-	543	WP_058951821.1	cysteine hydrolase family protein	-
LCH18_RS17710 (LCH18_17710)	1583..2467	-	885	WP_004733158.1	MBL fold metallo-hydrolase	-
LCH18_RS17715 (LCH18_17715)	2600..3577	+	978	WP_044109732.1	helix-turn-helix domain-containing protein	-
LCH18_RS17720 (LCH18_17720)	4490..5422	+	933	WP_053579251.1	IS5-like element ISAba12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8607	8607
	-	flank	IS/Tn	-	-	4490..5422	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T217838 WP_000286964.1 NZ_CP083949:285-587 [Acinetobacter johnsonii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T217838 NZ_CP114891:1443825-1443928 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT217838 WP_000985610.1 NZ_CP083949:588-869 [Acinetobacter johnsonii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT217838 NZ_CP114891:c1443966-1443695 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0A8TV94