Detailed information of TA system TA217832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 5219356..5221046
• Replicon: chromosome
• Accession: NZ_CP083938
• Organism: Comamonas thiooxydans strain CSUSB4

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LCH15_RS24130
• Protein ID: WP_224649248.1
• Coordinates: 5220168..5221046 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LCH15_RS24125
• Protein ID: WP_034394676.1
• Coordinates: 5219356..5220162 (-)
• Length: 269 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH15_RS24100 (LCH15_24100)	5214474..5215331	-	858	WP_034350821.1	IclR family transcriptional regulator	-
LCH15_RS24105 (LCH15_24105)	5215339..5216115	-	777	WP_034350824.1	SDR family oxidoreductase	-
LCH15_RS24110 (LCH15_24110)	5216430..5217188	-	759	WP_224649246.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
LCH15_RS24115 (LCH15_24115)	5217308..5218306	-	999	WP_034350829.1	tripartite tricarboxylate transporter substrate binding protein	-
LCH15_RS24120 (LCH15_24120)	5218513..5219313	-	801	WP_063662211.1	aspartate dehydrogenase	-
LCH15_RS24125 (LCH15_24125)	5219356..5220162	-	807	WP_034394676.1	IclR family transcriptional regulator	Antitoxin
LCH15_RS24130 (LCH15_24130)	5220168..5221046	-	879	WP_224649248.1	alpha/beta hydrolase	Toxin
LCH15_RS24135 (LCH15_24135)	5221055..5221588	-	534	WP_003072476.1	cupin domain-containing protein	-
LCH15_RS24140 (LCH15_24140)	5221850..5223526	-	1677	WP_034350834.1	long-chain-fatty-acid--CoA ligase	-
LCH15_RS24145 (LCH15_24145)	5223740..5224804	-	1065	WP_004340312.1	fructose-bisphosphate aldolase class II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (44-277)

Antitoxin

• Transcription regulator IclR, N-terminal (19-69)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 30609.13 Da
• Isoelectric Point: 6.1435

>T217832 WP_224649248.1 NZ_CP083938:c5221046-5220168 [Comamonas thiooxydans]
MHEDLLSLKLQLLQTGFPERLVKLPQGAQVAWREAGDASSDFAVVLLHGISSGAASWLDVALQLADKVRVLAWDAPGYGV
STPLAQSAPADADYAQALAQSLLVLGVRRCILVGHSLGALMAARLAAMAAPGLVEQLVLISPAGGYGAAAKAEQQSKVRE
GRLAALAEKGVAGLAAVIDQRLVSSEAPEVVRAWVRWNTARMQPQGYAQAVELLCGSDLAQAQGRLGMPVEVWVGEHDVV
TTPAACKSWSEMLGAHYGTLAAAGHASPVEQPMEVAHRLASLLNQSYCPNTS

Nucleotide

• Length: 879 bp

>T217832 NZ_CP114891:451156-451491 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 269 a.a.
• Molecular weight: 29245.19 Da
• Isoelectric Point: 5.3811

>AT217832 WP_034394676.1 NZ_CP083938:c5220162-5219356 [Comamonas thiooxydans]
MSETSEISTNEQDRYTVPALDRGLRLLACFGPAQPVWSAPELARSLELPRSTVFRMLTTLENSGYLQRSGTEYRLGLAVL
RLGYDYLSTQPLAQLAEPVLQALCDELGMTSNLALRDGTSVVYVARVTPSGAFQGAVRVGSRLPAHATVLGRALLHDMDG
AQLRSVFGGEELPQFSESTPRNVDELLRLLAEDRERGYAMGEGFYEPGVSSIAAPVRAADGRVVAGLAMAIPFTELGPEK
TQLWVQKVQQAAETLSAHLRQQHPDHLR

Nucleotide

• Length: 807 bp

>AT217832 NZ_CP114891:450908-451156 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA