Detailed information of TA system TA217831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2797671..2798272
• Replicon: chromosome
• Accession: NZ_CP083938
• Organism: Comamonas thiooxydans strain CSUSB4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCH15_RS12900
• Protein ID: WP_003055100.1
• Coordinates: 2797671..2798057 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7WTR2
• Locus tag: LCH15_RS12905
• Protein ID: WP_003055097.1
• Coordinates: 2798057..2798272 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH15_RS12875 (LCH15_12875)	2793081..2793446	+	366	WP_224648103.1	mercury resistance co-regulator MerD	-
LCH15_RS12880 (LCH15_12880)	2793443..2793679	+	237	WP_003055146.1	broad-spectrum mercury transporter MerE	-
LCH15_RS12885 (LCH15_12885)	2793745..2793957	+	213	WP_000993245.1	DUF3330 domain-containing protein	-
LCH15_RS12890 (LCH15_12890)	2794087..2794647	+	561	WP_003055107.1	recombinase family protein	-
LCH15_RS12895 (LCH15_12895)	2794650..2797618	+	2969	Protein_2493	Tn3 family transposase	-
LCH15_RS12900 (LCH15_12900)	2797671..2798057	-	387	WP_003055100.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCH15_RS12905 (LCH15_12905)	2798057..2798272	-	216	WP_003055097.1	DUF2191 domain-containing protein	Antitoxin
LCH15_RS12910 (LCH15_12910)	2798358..2799182	+	825	WP_003055094.1	SprT-like domain-containing protein	-
LCH15_RS12915 (LCH15_12915)	2799497..2802001	-	2505	WP_080554276.1	heavy metal translocating P-type ATPase	-
LCH15_RS12920 (LCH15_12920)	2802286..2803212	-	927	WP_224648105.1	copper homeostasis membrane protein CopD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2699919..2972029	272110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13816.18 Da
• Isoelectric Point: 5.9593

>T217831 WP_003055100.1 NZ_CP083938:c2798057-2797671 [Comamonas thiooxydans]
MPVSVLVDTSVWVRHFREADIHLQELLLQDSVLMHPMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLAFLEKNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLAELAAQLGVAYAIPTH

Nucleotide

• Length: 387 bp

>T217831 NZ_CP114891:320110-320517 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7448.46 Da
• Isoelectric Point: 6.2846

>AT217831 WP_003055097.1 NZ_CP083938:c2798272-2798057 [Comamonas thiooxydans]
MRSTFTIDDEVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGSAPDMPDVPRRGSAAEAEGSR

Nucleotide

• Length: 216 bp

>AT217831 NZ_CP114891:319863-320129 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A4Y8TDR8