Detailed information of TA system TA217830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2629334..2630882
• Replicon: chromosome
• Accession: NZ_CP083938
• Organism: Comamonas thiooxydans strain CSUSB4

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: LCH15_RS12115
• Protein ID: WP_224650965.1
• Coordinates: 2629584..2630882 (+)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: LCH15_RS12110
• Protein ID: WP_224650963.1
• Coordinates: 2629334..2629594 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH15_RS12090 (LCH15_12090)	2625762..2625908	-	147	WP_224648275.1	hypothetical protein	-
LCH15_RS12095 (LCH15_12095)	2625875..2626708	-	834	WP_224648276.1	IS5 family transposase	-
LCH15_RS12100 (LCH15_12100)	2626701..2627930	-	1230	WP_224650959.1	ATP-binding protein	-
LCH15_RS12105 (LCH15_12105)	2627983..2628711	-	729	WP_224650961.1	phospholipase D family protein	-
LCH15_RS12110 (LCH15_12110)	2629334..2629594	+	261	WP_224650963.1	helix-turn-helix transcriptional regulator	Antitoxin
LCH15_RS12115 (LCH15_12115)	2629584..2630882	+	1299	WP_224650965.1	type II toxin-antitoxin system HipA family toxin	Toxin
LCH15_RS12120 (LCH15_12120)	2631117..2631593	+	477	WP_224650968.1	GFA family protein	-
LCH15_RS12125 (LCH15_12125)	2631737..2633107	-	1371	WP_224650971.1	MFS transporter	-
LCH15_RS12130 (LCH15_12130)	2633871..2634227	-	357	WP_034355880.1	zinc ribbon domain-containing protein YjdM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-60)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 46951.99 Da
• Isoelectric Point: 8.1694

>T217830 WP_224650965.1 NZ_CP083938:2629584-2630882 [Comamonas thiooxydans]
MAHELEVWLFGRRIGSLSLVRGRLSFAYAADWLALPQATALSQSLLLQAERFDDHQTRPFFAGLLPEGQMRRLIAQQFQV
SGQNDFALLDHIGGDCAGAVTFLEPGQALAQTNGTGATGTAGDVEWLSDQKLLALLDELPRRPMLAGDDGLRLSLAGAQD
KLPVVVDESIPGAVRIGLPRNGAPSSHILKPAIHAVEDSVANEGFCMLLAQVMGLRPANARIQSVLGRSFLLVKRYDRVL
KDDAQGQAQLQRLHQEDFCQALGVVPELKYQNEGGPGLAQCFALARSVTRPSAPQVLRLLDGVIFNALIGNHDAHGKNFS
LLYGVRSPVLAPFYDLLATAIYPQLTPKMAMKIGSKYKFSELEARHWEQFAEESGLAKAATRKRLQQLANELPAAARKLQ
AAPPHGFAGSAVVEQVVQLIEQRSALTLRRLA

Nucleotide

• Length: 1299 bp

>T217830 NZ_CP114891:188514-188810 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 8824.03 Da
• Isoelectric Point: 5.5332

>AT217830 WP_224650963.1 NZ_CP083938:2629334-2629594 [Comamonas thiooxydans]
MSTIHTTDQLGAALRTARKQLGLTQPQLALAAGVGTRFIVDLEAGKPTVRLEHVLRVIDALGGELQLGGLPDTASEGVAE
GADHGA

Nucleotide

• Length: 261 bp

>AT217830 NZ_CP114891:188812-189207 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA