Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 342097..342619 Replicon plasmid pIncFIB_FIBK_HI1B
Accession NZ_CP083933
Organism Klebsiella pneumoniae strain BA4946

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag LCH45_RS27645 Protein ID WP_038991638.1
Coordinates 342335..342619 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag LCH45_RS27640 Protein ID WP_004181777.1
Coordinates 342097..342345 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH45_RS27620 (LCH45_27660) 337306..338127 - 822 WP_004181772.1 hypothetical protein -
LCH45_RS27625 (LCH45_27665) 338189..338542 - 354 WP_004181774.1 hypothetical protein -
LCH45_RS27630 (LCH45_27670) 338687..339673 - 987 WP_040120328.1 hypothetical protein -
LCH45_RS27635 (LCH45_27675) 340007..341806 - 1800 WP_043907032.1 ATP-dependent helicase -
LCH45_RS27640 (LCH45_27680) 342097..342345 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LCH45_RS27645 (LCH45_27685) 342335..342619 + 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCH45_RS27650 (LCH45_27690) 342636..342737 - 102 Protein_371 IS200/IS605 family transposase -
LCH45_RS27655 (LCH45_27695) 342773..343999 + 1227 WP_040120327.1 RNA-guided endonuclease TnpB family protein -
LCH45_RS27660 (LCH45_27700) 344271..344495 - 225 Protein_373 transposase -
LCH45_RS27665 (LCH45_27705) 344574..345002 - 429 WP_077256229.1 IS200/IS605 family transposase -
LCH45_RS27670 (LCH45_27710) 345038..346225 + 1188 WP_043907031.1 RNA-guided endonuclease TnpB family protein -
LCH45_RS27675 (LCH45_27715) 346270..346641 - 372 WP_044816264.1 hypothetical protein -
LCH45_RS27680 (LCH45_27720) 346638..346982 - 345 WP_014386518.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / blaTEM-1A / tet(D) / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 iucA / iucB / iucC / iucD / iutA / rmpA 1..421072 421072


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T217828 WP_038991638.1 NZ_CP083933:342335-342619 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T217828 NZ_CP114891:c79721-79257 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT217828 WP_004181777.1 NZ_CP083933:342097-342345 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT217828 NZ_CP114891:c80056-79721 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References