Detailed information of TA system TA217827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 281467..282218
• Replicon: plasmid pIncFIB_FIBK_HI1B
• Accession: NZ_CP083933
• Organism: Klebsiella pneumoniae strain BA4946

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LCH45_RS27315
• Protein ID: WP_048333033.1
• Coordinates: 281467..281949 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: LCH45_RS27320
• Protein ID: WP_004902250.1
• Coordinates: 281940..282218 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH45_RS27295 (LCH45_27330)	277866..278513	-	648	WP_014386537.1	EcsC family protein	-
LCH45_RS27300 (LCH45_27335)	278540..279295	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
LCH45_RS27305 (LCH45_27340)	279396..279788	-	393	WP_032442757.1	hypothetical protein	-
LCH45_RS27310 (LCH45_27345)	279893..280432	-	540	WP_004902239.1	hypothetical protein	-
LCH45_RS27315 (LCH45_27350)	281467..281949	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
LCH45_RS27320 (LCH45_27355)	281940..282218	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
LCH45_RS27325 (LCH45_27360)	282340..282552	-	213	WP_016946198.1	hypothetical protein	-
LCH45_RS27330 (LCH45_27365)	282660..283001	-	342	WP_032437747.1	hypothetical protein	-
LCH45_RS27335 (LCH45_27370)	283832..284290	-	459	WP_014386535.1	hypothetical protein	-
LCH45_RS27340 (LCH45_27375)	284943..285398	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
LCH45_RS27345 (LCH45_27380)	285470..285835	+	366	WP_004200999.1	mercuric ion transporter MerT	-
LCH45_RS27350 (LCH45_27385)	285851..286126	+	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
LCH45_RS27355 (LCH45_27390)	286154..286579	+	426	WP_000522996.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1A / tet(D) / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	iucA / iucB / iucC / iucD / iutA / rmpA	1..421072	421072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T217827 WP_048333033.1 NZ_CP083933:c281949-281467 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T217827 NZ_CP114886:6035913-6036182 [Endozoicomonas sp. GU-1]
ATGACCGATAAGCACCGACTGCTTTGCTGGTCACACAATGCCTGGAACGATTACCTGTACTGGCAGGAGCAGGACCGGAA
AACCCTGAAGCGCATCAATAAACTAATAACCGAGTGTCTGCGCGACCCATTTAAGGGTATTGGCAAACCCGAGCCGCTGA
AAGGTAACTGGCAGGGGTTCTGGTCACGGCGGATCAGCGATGAACACCGGCTGGTCTATCAGGCCACAGATGCCCGACTG
ATCATTGCTGCCTGCCGGTTTCACTACTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT217827 WP_004902250.1 NZ_CP083933:c282218-281940 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT217827 NZ_CP114886:6035666-6035923 [Endozoicomonas sp. GU-1]
ATGAAAGTCGTGTCCTATTCCGAAGCCCGGAACAATCTGAAATCCGTTCTTGATCAGGTGGCAGAGGATGCCAGTGAAAC
CCTGATTCATCGCCGTGACGGTCAGGATGCTGTTGTAATGTCGCTGGACAGCTACAACAGCCTGATGGAAACCCTGCACC
TGATGTCATCCCCGGCCAACGCCCAACACCTGAACGACTCCATTGAGCAGATCAGGACCGGGCAGGTCATGGAACGGGGA
TTGCTGGATGACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3