Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5260033..5260658 Replicon chromosome
Accession NZ_CP083932
Organism Klebsiella pneumoniae strain BA4946

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LCH45_RS25355 Protein ID WP_002882817.1
Coordinates 5260033..5260416 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LCH45_RS25360 Protein ID WP_004150355.1
Coordinates 5260416..5260658 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH45_RS25340 (5257399) 5257399..5258301 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LCH45_RS25345 (5258298) 5258298..5258933 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LCH45_RS25350 (5258930) 5258930..5259859 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LCH45_RS25355 (5260033) 5260033..5260416 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LCH45_RS25360 (5260416) 5260416..5260658 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LCH45_RS25365 (5260863) 5260863..5261780 + 918 WP_004181612.1 alpha/beta hydrolase -
LCH45_RS25370 (5261794) 5261794..5262735 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LCH45_RS25375 (5262780) 5262780..5263217 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LCH45_RS25380 (5263214) 5263214..5264074 - 861 WP_004146232.1 virulence factor BrkB family protein -
LCH45_RS25385 (5264068) 5264068..5264667 - 600 WP_267274010.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T217824 WP_002882817.1 NZ_CP083932:c5260416-5260033 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T217824 NZ_CP114886:5989018-5989395 [Endozoicomonas sp. GU-1]
ATGAAAGACCTGACCCCCGGTTTTTTTCCGTTTTTTGTGTGTGAACTTGAATATGTATCCAAATGGATACATAATTCCTT
AATGTACTTGCTCAAGACCACTGACCACTTCGAGAAGTGGCTGCACAAACTTAAAGACAAGCAGGGCAAGGCTCGAATCC
TGGCCTCTCTTAAATTGCTGGAGCAGGGACACATCGGGGACTGGAAATCAGTTGGAGGCAAAGTGTCAGAACTTCGGGTG
CACTACGGGGCAGGCTATAGGATCTACTTTGCCAGGCAAGAGGATGTCATCATCGTATTACTCAATGGTGGTGACAAGGC
CAGCCAGTCCAGAGACATTGAAAAAGCAAAAGCCATTTTAAAAAATCTGGAGGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT217824 WP_004150355.1 NZ_CP083932:c5260658-5260416 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT217824 NZ_CP114886:5989397-5989684 [Endozoicomonas sp. GU-1]
ATGAGCAGGAAGCTAAAGACCTTTGATGCATCAGAGTATCTTGACGACAGTGAAACTATTGCAGAGTACTTGAATGCAGT
CATCGAAACGGGTGACCAAGCTTTGCTCTTATCGGCTATTGGTGATATCGCCAAGGCAACAGGGATGCAGAAGATAGCCA
ATGACTCAGGCCTCGGTCGGGAAAGTTTATACAAAGCCCTGGCACCAGGTGCGAAACCAAGATTTGATACAGTGTTCAAA
ATTCTTCAGGCCCTGGGAGTGAGTTTGAAAATCAGCCCTCAGCATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References