Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4681011..4681821 Replicon chromosome
Accession NZ_CP083932
Organism Klebsiella pneumoniae strain BA4946

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LCH45_RS22680 Protein ID WP_004178461.1
Coordinates 4681011..4681544 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LCH45_RS22685 Protein ID WP_002887278.1
Coordinates 4681555..4681821 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH45_RS22675 (4679842) 4679842..4680963 + 1122 WP_002887282.1 cupin domain-containing protein -
LCH45_RS22680 (4681011) 4681011..4681544 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LCH45_RS22685 (4681555) 4681555..4681821 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LCH45_RS22690 (4681924) 4681924..4683357 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
LCH45_RS22695 (4683347) 4683347..4684030 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LCH45_RS22700 (4684202) 4684202..4685587 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
LCH45_RS22705 (4685605) 4685605..4685949 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T217821 WP_004178461.1 NZ_CP083932:c4681544-4681011 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T217821 NZ_CP114886:5239428-5239712 [Endozoicomonas sp. GU-1]
GTGTCCACAGAAATATTCGATGACTGGTATCGCCAGCTCAAAGACCGTACCGCAGCCAAAGCTATTGCCCTGCGTATTAC
CAGAGCCAGGGCTGGGAATTTGGGCGACACCAGGATGATTGGTGGTGGTATCAGTGAGATGCGGGTGTTTATTGGCAAAG
GTTATCGTTTATATTTCACGATCCGTAACCGCCAGCTGGTTATTCTTCTTTGTGGTGGCGATAAATCCAGCCAAAGTAGA
GACATTAAAAAAGCGAAATCACTATTGGATCAATTGGAGGATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT217821 WP_002887278.1 NZ_CP083932:c4681821-4681555 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT217821 NZ_CP114886:5239074-5239295 [Endozoicomonas sp. GU-1]
ATGAAAGCCAGTGATATTGATAAAAAATTCGACGATGGCGAAGACGTACTGGAATTCTTCGACACCGACCATCCTCAACG
GCCCAACCTTGCAGCCAAAAGGGTCAACATTGACTTCCCTGCATGGGTTGTCAATGAGCTGGATAAAGAAGCCAGCGCAA
TCGGCATCAGCCGTCAGGCGCTGATTAAAACGTGGATTGTTGACCGGCTCAGGCAGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References