Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | omega-epsilon-zeta/zeta-epsilon |
| Location | 46227..47364 | Replicon | plasmid pV1164-2 |
| Accession | NZ_CP083922 | ||
| Organism | Enterococcus faecium isolate V1164 | ||
Toxin (Protein)
| Gene name | zeta | Uniprot ID | P0A4M2 |
| Locus tag | LCG90_RS14680 | Protein ID | WP_002332783.1 |
| Coordinates | 46227..47090 (-) | Length | 288 a.a. |
Antitoxin (Protein)
| Gene name | epsilon | Uniprot ID | Q9AL19 |
| Locus tag | LCG90_RS14685 | Protein ID | WP_002326825.1 |
| Coordinates | 47092..47364 (-) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCG90_RS14645 (LCG90_14665) | 41616..42524 | - | 909 | WP_001255866.1 | aminoglycoside nucleotidyltransferase ANT(6)-Ia | - |
| LCG90_RS14650 (LCG90_14670) | 42557..43291 | - | 735 | WP_000662263.1 | class I SAM-dependent methyltransferase | - |
| LCG90_RS14655 (LCG90_14675) | 43272..44141 | - | 870 | WP_000233000.1 | nucleotidyltransferase domain-containing protein | - |
| LCG90_RS14660 (LCG90_14680) | 44156..44380 | - | 225 | WP_000205227.1 | helix-turn-helix transcriptional regulator | - |
| LCG90_RS14665 (LCG90_14685) | 44523..44714 | - | 192 | Protein_54 | recombinase family protein | - |
| LCG90_RS14670 (LCG90_14690) | 44769..45449 | - | 681 | WP_001067799.1 | IS6-like element IS1216 family transposase | - |
| LCG90_RS14675 (LCG90_14695) | 45524..45664 | - | 141 | Protein_56 | IS6 family transposase | - |
| LCG90_RS14680 (LCG90_14705) | 46227..47090 | - | 864 | WP_002332783.1 | zeta toxin family protein | Toxin |
| LCG90_RS14685 (LCG90_14710) | 47092..47364 | - | 273 | WP_002326825.1 | antitoxin | Antitoxin |
| LCG90_RS14690 (LCG90_14715) | 47382..47597 | - | 216 | WP_001835296.1 | peptide-binding protein | - |
| LCG90_RS14695 (LCG90_14720) | 47689..48585 | - | 897 | WP_002326827.1 | ParA family protein | - |
| LCG90_RS14705 (LCG90_14730) | 50107..50367 | - | 261 | Protein_62 | topoisomerase DNA-binding C4 zinc finger domain-containing protein | - |
| LCG90_RS14710 (LCG90_14735) | 50537..51274 | - | 738 | WP_001038795.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | cat(pC221) / VanHAX / ant(6)-Ia / erm(B) | - | 1..52056 | 52056 | |
| - | inside | IScluster/Tn | ant(6)-Ia / erm(B) | - | 35558..51274 | 15716 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 288 a.a. Molecular weight: 32675.27 Da Isoelectric Point: 7.3939
>T217803 WP_002332783.1 NZ_CP083922:c47090-46227 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI
Download Length: 864 bp
>T217803 NZ_CP114853:c4677892-4677551 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10720.17 Da Isoelectric Point: 4.4865
>AT217803 WP_002326825.1 NZ_CP083922:c47364-47092 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA
Download Length: 273 bp
>AT217803 NZ_CP114853:c4678254-4677913 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | P0A4M2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M2AF93 |