Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 46227..47364 Replicon plasmid pV1164-2
Accession NZ_CP083922
Organism Enterococcus faecium isolate V1164

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag LCG90_RS14680 Protein ID WP_002332783.1
Coordinates 46227..47090 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag LCG90_RS14685 Protein ID WP_002326825.1
Coordinates 47092..47364 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCG90_RS14645 (LCG90_14665) 41616..42524 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
LCG90_RS14650 (LCG90_14670) 42557..43291 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
LCG90_RS14655 (LCG90_14675) 43272..44141 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein -
LCG90_RS14660 (LCG90_14680) 44156..44380 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
LCG90_RS14665 (LCG90_14685) 44523..44714 - 192 Protein_54 recombinase family protein -
LCG90_RS14670 (LCG90_14690) 44769..45449 - 681 WP_001067799.1 IS6-like element IS1216 family transposase -
LCG90_RS14675 (LCG90_14695) 45524..45664 - 141 Protein_56 IS6 family transposase -
LCG90_RS14680 (LCG90_14705) 46227..47090 - 864 WP_002332783.1 zeta toxin family protein Toxin
LCG90_RS14685 (LCG90_14710) 47092..47364 - 273 WP_002326825.1 antitoxin Antitoxin
LCG90_RS14690 (LCG90_14715) 47382..47597 - 216 WP_001835296.1 peptide-binding protein -
LCG90_RS14695 (LCG90_14720) 47689..48585 - 897 WP_002326827.1 ParA family protein -
LCG90_RS14705 (LCG90_14730) 50107..50367 - 261 Protein_62 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LCG90_RS14710 (LCG90_14735) 50537..51274 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid cat(pC221) / VanHAX / ant(6)-Ia / erm(B) - 1..52056 52056
- inside IScluster/Tn ant(6)-Ia / erm(B) - 35558..51274 15716


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T217803 WP_002332783.1 NZ_CP083922:c47090-46227 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T217803 NZ_CP114853:c4677892-4677551 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT217803 WP_002326825.1 NZ_CP083922:c47364-47092 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT217803 NZ_CP114853:c4678254-4677913 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References