Detailed information of TA system TA217802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 43272..44380
• Replicon: plasmid pV1164-2
• Accession: NZ_CP083922
• Organism: Enterococcus faecium isolate V1164

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LCG90_RS14655
• Protein ID: WP_000233000.1
• Coordinates: 43272..44141 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LCG90_RS14660
• Protein ID: WP_000205227.1
• Coordinates: 44156..44380 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCG90_RS14620 (LCG90_14640)	38750..39262	-	513	WP_000774078.1	hypothetical protein	-
LCG90_RS14625 (LCG90_14645)	39269..39787	-	519	WP_000357244.1	YfbU family protein	-
LCG90_RS14630 (LCG90_14650)	39949..40161	-	213	WP_002349227.1	hypothetical protein	-
LCG90_RS14635 (LCG90_14655)	40370..41050	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LCG90_RS14640 (LCG90_14660)	41139..41619	-	481	Protein_49	GNAT family N-acetyltransferase	-
LCG90_RS14645 (LCG90_14665)	41616..42524	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LCG90_RS14650 (LCG90_14670)	42557..43291	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LCG90_RS14655 (LCG90_14675)	43272..44141	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LCG90_RS14660 (LCG90_14680)	44156..44380	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LCG90_RS14665 (LCG90_14685)	44523..44714	-	192	Protein_54	recombinase family protein	-
LCG90_RS14670 (LCG90_14690)	44769..45449	-	681	WP_001067799.1	IS6-like element IS1216 family transposase	-
LCG90_RS14675 (LCG90_14695)	45524..45664	-	141	Protein_56	IS6 family transposase	-
LCG90_RS14680 (LCG90_14705)	46227..47090	-	864	WP_002332783.1	zeta toxin family protein	-
LCG90_RS14685 (LCG90_14710)	47092..47364	-	273	WP_002326825.1	antitoxin	-
LCG90_RS14690 (LCG90_14715)	47382..47597	-	216	WP_001835296.1	peptide-binding protein	-
LCG90_RS14695 (LCG90_14720)	47689..48585	-	897	WP_002326827.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cat(pC221) / VanHAX / ant(6)-Ia / erm(B)	-	1..52056	52056
	-	inside	IScluster/Tn	ant(6)-Ia / erm(B)	-	35558..51274	15716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T217802 WP_000233000.1 NZ_CP083922:c44141-43272 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T217802 NZ_CP114853:c4647420-4646887 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT217802 WP_000205227.1 NZ_CP083922:c44380-44156 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT217802 NZ_CP114853:c4647697-4647431 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: R3JIN1