Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 47596..48203 Replicon plasmid pV1164-1
Accession NZ_CP083921
Organism Enterococcus faecium isolate V1164

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag LCG90_RS13450 Protein ID WP_002321032.1
Coordinates 47853..48203 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag LCG90_RS13445 Protein ID WP_002287514.1
Coordinates 47596..47859 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCG90_RS13400 (LCG90_13415) 42632..43531 + 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LCG90_RS13405 (LCG90_13420) 43534..44019 + 486 WP_002296241.1 single-stranded DNA-binding protein -
LCG90_RS13410 (LCG90_13425) 44385..44645 + 261 WP_002296240.1 hypothetical protein -
LCG90_RS13415 (LCG90_13430) 45086..45292 + 207 WP_002296239.1 hypothetical protein -
LCG90_RS13420 (LCG90_13435) 45292..45543 + 252 WP_002296238.1 hypothetical protein -
LCG90_RS13425 (LCG90_13440) 45558..45995 + 438 WP_002296237.1 hypothetical protein -
LCG90_RS13430 (LCG90_13445) 45988..46695 + 708 WP_002353391.1 hypothetical protein -
LCG90_RS13435 (LCG90_13450) 46860..47060 + 201 WP_002330566.1 hypothetical protein -
LCG90_RS13440 (LCG90_13455) 47076..47255 + 180 WP_002305761.1 hypothetical protein -
LCG90_RS13445 (LCG90_13460) 47596..47859 + 264 WP_002287514.1 hypothetical protein Antitoxin
LCG90_RS13450 (LCG90_13465) 47853..48203 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LCG90_RS13455 (LCG90_13470) 48227..48841 - 615 WP_010729795.1 recombinase family protein -
LCG90_RS13460 (LCG90_13475) 49155..49577 + 423 WP_002288787.1 hypothetical protein -
LCG90_RS13465 (LCG90_13480) 49587..49790 + 204 WP_002299573.1 hypothetical protein -
LCG90_RS13470 (LCG90_13485) 50001..50585 + 585 WP_002299575.1 recombinase family protein -
LCG90_RS13475 (LCG90_13490) 50774..51454 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -
LCG90_RS13480 (LCG90_13495) 51508..52218 - 711 WP_010729797.1 GntR family transcriptional regulator -
LCG90_RS13485 (LCG90_13500) 52202..52936 - 735 WP_010729798.1 GntR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..213279 213279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T217800 WP_002321032.1 NZ_CP083921:47853-48203 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T217800 NZ_CP114853:3973741-3973959 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT217800 WP_002287514.1 NZ_CP083921:47596-47859 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT217800 NZ_CP114853:3973340-3973714 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References