Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Xre-MNT/HTH_26(antitoxin) |
| Location | 41456..42564 | Replicon | plasmid pV1225-2 |
| Accession | NZ_CP083914 | ||
| Organism | Enterococcus faecium isolate V1225 | ||
Toxin (Protein)
| Gene name | MNTss | Uniprot ID | R3JIN1 |
| Locus tag | LCG89_RS14725 | Protein ID | WP_000233000.1 |
| Coordinates | 41456..42325 (-) | Length | 290 a.a. |
Antitoxin (Protein)
| Gene name | Xress | Uniprot ID | - |
| Locus tag | LCG89_RS14730 | Protein ID | WP_000205227.1 |
| Coordinates | 42340..42564 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCG89_RS14690 (LCG89_14690) | 36934..37446 | - | 513 | WP_000774078.1 | hypothetical protein | - |
| LCG89_RS14695 (LCG89_14695) | 37453..37971 | - | 519 | WP_000357244.1 | YfbU family protein | - |
| LCG89_RS14700 (LCG89_14700) | 38133..38345 | - | 213 | WP_002349227.1 | hypothetical protein | - |
| LCG89_RS14705 (LCG89_14705) | 38554..39234 | + | 681 | WP_001015311.1 | IS6-like element IS1216 family transposase | - |
| LCG89_RS14710 (LCG89_14710) | 39323..39803 | - | 481 | Protein_45 | GNAT family N-acetyltransferase | - |
| LCG89_RS14715 (LCG89_14715) | 39800..40708 | - | 909 | WP_001255866.1 | aminoglycoside nucleotidyltransferase ANT(6)-Ia | - |
| LCG89_RS14720 (LCG89_14720) | 40741..41475 | - | 735 | WP_000662263.1 | class I SAM-dependent methyltransferase | - |
| LCG89_RS14725 (LCG89_14725) | 41456..42325 | - | 870 | WP_000233000.1 | nucleotidyltransferase domain-containing protein | Toxin |
| LCG89_RS14730 (LCG89_14730) | 42340..42564 | - | 225 | WP_000205227.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| LCG89_RS14735 (LCG89_14735) | 42707..42898 | - | 192 | Protein_50 | recombinase family protein | - |
| LCG89_RS14740 (LCG89_14740) | 42953..43633 | - | 681 | WP_001067799.1 | IS6-like element IS1216 family transposase | - |
| LCG89_RS14745 (LCG89_14745) | 43708..43848 | - | 141 | Protein_52 | IS6 family transposase | - |
| LCG89_RS14750 (LCG89_14750) | 43876..44019 | - | 144 | WP_154641890.1 | hypothetical protein | - |
| LCG89_RS14755 (LCG89_14755) | 44411..45274 | - | 864 | WP_002332783.1 | zeta toxin family protein | - |
| LCG89_RS14760 (LCG89_14760) | 45276..45548 | - | 273 | WP_002326825.1 | antitoxin | - |
| LCG89_RS14765 (LCG89_14765) | 45566..45781 | - | 216 | WP_001835296.1 | peptide-binding protein | - |
| LCG89_RS14770 (LCG89_14770) | 45873..46769 | - | 897 | WP_002326827.1 | ParA family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | erm(B) / cat(pC221) / VanHAX / ant(6)-Ia | - | 1..48157 | 48157 | |
| - | inside | IScluster/Tn | ant(6)-Ia | - | 33742..48010 | 14268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 290 a.a. Molecular weight: 32926.57 Da Isoelectric Point: 4.8781
>T217797 WP_000233000.1 NZ_CP083914:c42325-41456 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
Download Length: 870 bp
>T217797 NZ_CP114853:1928649-1928751 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 75 a.a. Molecular weight: 8365.68 Da Isoelectric Point: 4.5510
>AT217797 WP_000205227.1 NZ_CP083914:c42564-42340 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
Download Length: 225 bp
>AT217797 NZ_CP114853:c1928758-1928613 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|