Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2228922..2229493 Replicon chromosome
Accession NZ_CP083912
Organism Enterococcus faecium isolate V1225

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LCG89_RS10900 Protein ID WP_002286801.1
Coordinates 2228922..2229263 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LCG89_RS10905 Protein ID WP_002323011.1
Coordinates 2229263..2229493 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCG89_RS10875 (LCG89_10875) 2224342..2225172 + 831 WP_002286789.1 manganese catalase family protein -
LCG89_RS10880 (LCG89_10880) 2225416..2225665 - 250 Protein_2133 transposase -
LCG89_RS10885 (LCG89_10885) 2225856..2226050 - 195 WP_002297028.1 hypothetical protein -
LCG89_RS10890 (LCG89_10890) 2226555..2226740 - 186 WP_002304207.1 hypothetical protein -
LCG89_RS10895 (LCG89_10895) 2226951..2228123 + 1173 WP_002323245.1 IS256-like element IS1542 family transposase -
LCG89_RS10900 (LCG89_10900) 2228922..2229263 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LCG89_RS10905 (LCG89_10905) 2229263..2229493 - 231 WP_002323011.1 hypothetical protein Antitoxin
LCG89_RS10910 (LCG89_10910) 2229780..2230118 + 339 WP_002286804.1 hypothetical protein -
LCG89_RS10915 (LCG89_10915) 2230323..2230885 - 563 Protein_2140 SOS response-associated peptidase -
LCG89_RS10920 (LCG89_10920) 2231617..2232198 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
LCG89_RS10925 (LCG89_10925) 2232381..2233007 - 627 WP_002286816.1 cysteine hydrolase -
LCG89_RS10930 (LCG89_10930) 2233029..2234360 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2226951..2228123 1172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T217794 WP_002286801.1 NZ_CP083912:c2229263-2228922 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T217794 NZ_CP114853:323162-323530 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT217794 WP_002323011.1 NZ_CP083912:c2229493-2229263 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT217794 NZ_CP114853:322944-323165 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References