Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 93498..94045 Replicon plasmid unnamed2
Accession NZ_CP083890
Organism Escherichia coli strain NDM1

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag LCE25_RS25035 Protein ID WP_001384452.1
Coordinates 93498..93776 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag LCE25_RS25040 Protein ID WP_000079941.1
Coordinates 93776..94045 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE25_RS25010 (89437) 89437..90297 - 861 WP_000493383.1 EamA family transporter -
LCE25_RS25015 (90306) 90306..90920 - 615 Protein_97 transposase -
LCE25_RS25020 (90915) 90915..91040 + 126 Protein_98 MerR family transcriptional regulator -
LCE25_RS25025 (91119) 91119..92129 + 1011 WP_224446622.1 IS110-like element IS4321 family transposase -
LCE25_RS25030 (92610) 92610..93458 - 849 WP_001057996.1 3'-5' exonuclease -
LCE25_RS25035 (93498) 93498..93776 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
LCE25_RS25040 (93776) 93776..94045 - 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
LCE25_RS25045 (94100) 94100..94258 - 159 WP_000841008.1 hypothetical protein -
LCE25_RS25050 (94954) 94954..95985 - 1032 WP_000907873.1 plasmid replication initiator RepA -
LCE25_RS25055 (96991) 96991..97221 + 231 WP_228772074.1 ash family protein -
LCE25_RS25060 (97139) 97139..97426 + 288 WP_000074849.1 conjugal transfer protein TraA -
LCE25_RS25065 (97868) 97868..98401 + 534 WP_001303319.1 transcription termination/antitermination NusG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) - 1..137647 137647


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T217778 WP_001384452.1 NZ_CP083890:c93776-93498 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T217778 NZ_CP114791:2705715-2705987 [Sphingomonas sp. NIBR02145]
ATGCTCCCGCACCTCTGGCACCCTGAGGCGCTGGAGGATCTTGAGACCGTCATCGATTTCATCGAAGCACGTAACCCGTC
AGCCGCGCGGCGGATCGGCGATGCCATCCGCAGCACCGCGGACCAGTTGCCCGCGCATTCCCATGCCTATCGGCCCGGCC
GTGTTCCCGGCACCCGCGAGGCGGTTGTCCATCCCAACTACATCCTGATCTATCGCGTCGCCGAGATCATCGAGGTGATG
GCCGTCTTCCACGCCCGCCAGCTTTATCCCTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT217778 WP_000079941.1 NZ_CP083890:c94045-93776 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT217778 NZ_CP114791:2705539-2705715 [Sphingomonas sp. NIBR02145]
ATGGCAAAGCTCTCGCCCATCGTCTCGGAATTCGCCTCCACCGAGGAGGAGGAAGCCTATGATGCGTGGTTCCGCGCCAA
GGTCGAAGCCCGGCTGGCAGACAAGGCGCCCGGCATCCCGCATGAAGAAGTCATGGCAAGGATGCAGGCCATCCTCGATC
GTCACGCGCAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References