Detailed information of TA system TA217773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 183636..184279
• Replicon: plasmid unnamed1
• Accession: NZ_CP083889
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: LCE25_RS24360
• Protein ID: WP_001034044.1
• Coordinates: 183863..184279 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: LCE25_RS24355
• Protein ID: WP_088569139.1
• Coordinates: 183636..183866 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS24335 (179198)	179198..179887	-	690	WP_001513524.1	RES family NAD+ phosphorylase	-
LCE25_RS24340 (180306)	180306..180536	+	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	-
LCE25_RS24345 (180533)	180533..180949	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	-
LCE25_RS24350 (181111)	181111..183249	-	2139	WP_001513523.1	AAA family ATPase	-
LCE25_RS24355 (183636)	183636..183866	+	231	WP_088569139.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LCE25_RS24360 (183863)	183863..184279	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCE25_RS24365 (184354)	184354..185919	+	1566	WP_001128474.1	AAA family ATPase	-
LCE25_RS24370 (185904)	185904..186926	+	1023	WP_032174242.1	helicase UvrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..219996	219996
	-	flank	IS/Tn	-	-	187180..187683	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T217773 WP_001034044.1 NZ_CP083889:183863-184279 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T217773 NZ_CP114774:207161-207448 [Raoultella planticola]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAGCTGGATAAGGCCATTCAGCAGCAGTTTGC
GAAGAAACTGAAGAAGTGCTGTGACGAACCACATATCCCTTCAGCAAAGCTGAGAGGAATAAAGGACTGCTACAAAATTA
AACTTCGTGCATCAGGTTTTCGCCTGGTGTATCAGGTGATAGATGATCAATTAATTATCGCGGTTGTTGCTGTTGGAAAG
CGTGAACGCAGTGACGTGTACAATCTGGCCAGCGAAAGGATGAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8761.89 Da
• Isoelectric Point: 4.3895

>AT217773 WP_088569139.1 NZ_CP083889:183636-183866 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKAEPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT217773 NZ_CP114774:206913-207164 [Raoultella planticola]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTAAAAAAAAATCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCGATTCTGAACCGTAACCAGCCGGCTTTTTACTGCATCCCGGCTGAACTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTATCAGAACGCAGTAACCAGCAGCTGCTTGACGTGGATCTGGAT
ACTTATCTATGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1