Detailed information of TA system TA217767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 119596..120221
• Replicon: plasmid unnamed1
• Accession: NZ_CP083889
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCE25_RS23965
• Protein ID: WP_000911313.1
• Coordinates: 119823..120221 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: LCE25_RS23960
• Protein ID: WP_000450520.1
• Coordinates: 119596..119823 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS23960 (119596)	119596..119823	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LCE25_RS23965 (119823)	119823..120221	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCE25_RS23970 (120230)	120230..122428	-	2199	WP_219891973.1	type IV conjugative transfer system coupling protein TraD	-
LCE25_RS23975 (122681)	122681..123412	-	732	WP_130570567.1	conjugal transfer complement resistance protein TraT	-
LCE25_RS23980 (123444)	123444..123941	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..219996	219996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T217767 WP_000911313.1 NZ_CP083889:119823-120221 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T217767 NZ_CP114772:c2917231-2915900 [Raoultella planticola]
ATGGCTACTCTCGTGACATGGATGAACAATGAGCGAGTCGGCGAACTTATCAAGCAGGCGAACGGCGCCCATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCTCGCGCCAGGCCGCTCTCTCTGTCGCTCCCGCTGCAGTACGGTAACATCACCG
ACGATGCGGTGTTTAACTATTTTGACAACCTGCTGCCGGACAGCCCGCGGGTGCGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCTTTTGATCTGCTGGCCGAGATCGGACGCGACAGCGTCGGGGCGGTCACGTTACTCCCGCCAGG
GGAAAATGCAACCCGGGGGGCGTTGAGCTGGGAGGCGCTGGATAGCCACGCCCTGGATGCGCTGCTGACGGCCTACCAGT
CGGATATACCGCTCGGGATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATACCGACGGGGACCACGCCGACCACCCATATCATTAAACTGCCTATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTAGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCGCGCCAGCATTATCCAGACCGCCAGCATCAGGGCGCTGGCGGTTGAGCGTTTCGACCGTCGTTGG
GCGCAGGAGAAGACGGTGCTGCTGCGTTTACCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCGATTATGGCTTTTCTGGTTGGATCCAGCGAGGCGCTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCGAAAAACTTCTCGATCTTCCTCCAG
CCGGGCGGCAGCTATCGCTTAACCCCATTCTACGACATTATCTCCGCGTTTCCGCTGCTTGGCGGGAAGGGGCTGCACCT
GAGCGATCTCCGGCTGTCAATGAGCCTGAAGGCCAGTAAAGGGCGCAAAACGGAGATCCAGACGCTCTATCCGCGTCATT
TCCTCGCCACGGCGAAAGAGGTGGGGTTTGCCGAGGAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CACGCGGTTGCTGCGGTAAAGGAAACTTTACCGCATGACTTTTCACCACCGGTGTATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTTACCGTCGGGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT217767 WP_000450520.1 NZ_CP083889:119596-119823 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT217767 NZ_CP114772:c2917503-2917231 [Raoultella planticola]
ATGAGCCCTTCACCAGGAATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGACCTGGCTCAGCGTATGGGGATTAAACAGGCTACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCAACGAT
AGCAGTGATAACCCACAGGATCTGGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2