Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4309479..4309976 Replicon chromosome
Accession NZ_CP083888
Organism Escherichia coli strain NDM1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LCE25_RS20795 Protein ID WP_001173343.1
Coordinates 4309479..4309652 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag LCE25_RS20800 Protein ID WP_001425728.1
Coordinates 4309680..4309976 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE25_RS20785 (4306149) 4306149..4307606 + 1458 WP_224446584.1 dipeptide/tripeptide permease DtpC -
LCE25_RS20790 (4307843) 4307843..4309360 + 1518 WP_001295074.1 lysine--tRNA ligase -
LCE25_RS20795 (4309479) 4309479..4309652 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LCE25_RS20800 (4309680) 4309680..4309976 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCE25_RS20805 (4310203) 4310203..4310475 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LCE25_RS20810 (4310487) 4310487..4310717 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
LCE25_RS20815 (4310898) 4310898..4312529 + 1632 WP_001216487.1 sensor histidine kinase -
LCE25_RS20820 (4312526) 4312526..4313245 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T217761 WP_001173343.1 NZ_CP083888:c4309652-4309479 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T217761 NZ_CP114771:5106707-5107219 [Endozoicomonas sp. GU-1]
GTGGAACTCGATAAAGGTATCCACGATAGGGCATCATTTGATTGTGGTGAAACGGAACTTAATTTGTTCATCCAGACTCA
AGCCTCAAAACATATGCAGGCAGGTATCAGTCGTACAATGGTTCTGCCTGCATCAAACCCTTTTCCAAATCAAAAAATTC
CAATTTGTGCATTTTACAGCATTACGCCAGGCTCAATCTGTCGAGACACTTTACCTGAAGCTCTGGTAAAGAAGCTGCCA
CGGTATCCTGTTCCTGTATTTTTGATCGCACAGCTTGCGGTTCATCGAGAATTTCAGGGCGAGGGGTTAGGAAAAATCTG
CCTGGTAAATGCCTTGAAATACCTGTGGGAAATAAACGCTCATATGCGTGCATACGCCATTATTGTTGATTGTTTAACCG
ATGCCGCAGAGCAGTTTTATGCAAAATATGGATTTGAAGTTCTGTGTGAACATAATGGTAAAGTTCGCATGTTTCTGCCC
ATGAAAACAGTAGCCCGGCTTTTTAGTTCGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT217761 WP_001425728.1 NZ_CP083888:c4309976-4309680 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT217761 NZ_CP114771:5106420-5106689 [Endozoicomonas sp. GU-1]
ATGGCTAATGCACGCCTGGACCTGAGACTTGATGAGGAAATCAAGGCTAAGGCAGAGAAGGCTTCAGCTCTACTGGGACT
GAAAAGCTTGACCGAATACGTGGTCAGGCTTATGGATGAAGACGCTACACAGGTTATTTCTGAATATGAAAAGATGACAG
TTGAAAACGATGTCTTTGATCGCTTTACAGAAGCCTGCGAAAAGGCAAAGGCACCAAATAAGGCTTTGCTGGCTGCCCTG
ACATTTACCGAAGAGAAAAGTGTCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References