Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3527400..3528018 Replicon chromosome
Accession NZ_CP083888
Organism Escherichia coli strain NDM1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LCE25_RS17030 Protein ID WP_001291435.1
Coordinates 3527800..3528018 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LCE25_RS17025 Protein ID WP_000344800.1
Coordinates 3527400..3527774 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE25_RS17015 (3522489) 3522489..3523682 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LCE25_RS17020 (3523705) 3523705..3526854 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LCE25_RS17025 (3527400) 3527400..3527774 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LCE25_RS17030 (3527800) 3527800..3528018 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LCE25_RS17035 (3528190) 3528190..3528741 + 552 WP_000102568.1 maltose O-acetyltransferase -
LCE25_RS17040 (3528857) 3528857..3529327 + 471 WP_000136192.1 YlaC family protein -
LCE25_RS17045 (3529491) 3529491..3531041 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LCE25_RS17050 (3531083) 3531083..3531436 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LCE25_RS17060 (3531815) 3531815..3532126 + 312 WP_000409911.1 MGMT family protein -
LCE25_RS17065 (3532157) 3532157..3532729 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217759 WP_001291435.1 NZ_CP083888:3527800-3528018 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T217759 NZ_CP114771:3485191-3485448 [Endozoicomonas sp. GU-1]
ATGAAAGTCGTGTCCTATTCCGAAGCCCGGAACAATCTGAAATCCGTTCTTGATCAGGTGGCAGAGGATGCCAGTGAAAC
CCTGATTCATCGCCGTGACGGTCAGGATGCTGTTGTAATGTCGCTGGACAGCTACAACAGCCTGATGGAAACCCTGCACC
TGATGTCATCCCCGGCCAACGCCCAACACCTGAACGACTCCATTGAGCAGATCAGGACCGGGCAGGTCATGGAACGGGGA
TTGCTGGATGACCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT217759 WP_000344800.1 NZ_CP083888:3527400-3527774 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT217759 NZ_CP114771:3485438-3485707 [Endozoicomonas sp. GU-1]
ATGACCGATAAGCACCGACTGCTTTGCTGGTCACACAATGCCTGGAACGATTACCTGTACTGGCAGGAGCAGGACCGGAA
AACCCTGAAGCGCATCAATAAACTAATAACCGAGTGTCTGCGCGACCCATTTAAGGGTATTGGCAAACCCGAGCCGCTGA
AAGGTAACTGGCAGGGGTTCTGGTCACGGCGGATCAGCGATGAACACCGGCTGGTCTATCAGGCCACAGATGCCCGACTG
ATCATTGCTGCCTGCCGGTTTCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References