Detailed information of TA system TA217748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1324280..1324905
• Replicon: chromosome
• Accession: NZ_CP083888
• Organism: Escherichia coli strain NDM1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCE25_RS06430
• Protein ID: WP_000911330.1
• Coordinates: 1324507..1324905 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LCE25_RS06425
• Protein ID: WP_000450524.1
• Coordinates: 1324280..1324507 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE25_RS06400 (1320083)	1320083..1320553	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LCE25_RS06405 (1320553)	1320553..1321125	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LCE25_RS06410 (1321271)	1321271..1322149	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LCE25_RS06415 (1322166)	1322166..1323200	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LCE25_RS06420 (1323413)	1323413..1324126	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LCE25_RS06425 (1324280)	1324280..1324507	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LCE25_RS06430 (1324507)	1324507..1324905	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCE25_RS06435 (1325052)	1325052..1325915	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
LCE25_RS06440 (1325930)	1325930..1327945	+	2016	WP_069354268.1	tRNA cytosine(34) acetyltransferase TmcA	-
LCE25_RS06445 (1328019)	1328019..1328717	+	699	WP_000679812.1	esterase	-
LCE25_RS06450 (1328827)	1328827..1329027	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T217748 WP_000911330.1 NZ_CP083888:1324507-1324905 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T217748 NZ_CP114771:c271008-270721 [Endozoicomonas sp. GU-1]
ATGACCTATGAGCTGGATTTTTCTGCACTGGCATGGAAAGAATGGCAGAAACTTAACTCCACAGTGAGAGAACAGTTCAA
AGCCAAGCTGCGTAAACGCTTGGAGAACCCTAAAGTTTCCAAAGATAAACTGTCCGGCCAGAAAGACTGCTACAAAATCA
AACTGCGAAATTCGGGCTATCGGTTGGTTTATCAGGTCTTGGACGACGTTGTTGTAGTATTTGTGATCAGTGTCGGTAAA
CGAGAGCGAAGCGAAGCCTATAAAGCCGCTCAAAAACGACTACGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT217748 WP_000450524.1 NZ_CP083888:1324280-1324507 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT217748 NZ_CP114771:c271252-270998 [Endozoicomonas sp. GU-1]
ATGGCCTCAGTCCATCCTATTCTCGCTGATGTCAGTGCCGGGATATCCGAACTCAAAAAAAACCCGATGGCCGTTCTGAA
AGAAGCCAATGGTGAAACCGTGGCCATCCTGAATCGCAATCAGCCCGTGTTTTATGCAGTGCCAGCCCATGTTTATGAAG
CTATGCTCGATGCTCTGGATGACCTGGAACTCGCAGCCATCGTTGAAGAACGCAAAAATGATGAGCGGATTCGGGTAAAT
ATTGATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33