Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 868682..869336 Replicon chromosome
Accession NZ_CP083888
Organism Escherichia coli strain NDM1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LCE25_RS04245 Protein ID WP_000244777.1
Coordinates 868929..869336 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LCE25_RS04240 Protein ID WP_000354046.1
Coordinates 868682..868948 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE25_RS04215 (863970) 863970..864713 + 744 WP_000951948.1 SDR family oxidoreductase -
LCE25_RS04220 (864770) 864770..866203 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LCE25_RS04225 (866248) 866248..866559 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LCE25_RS04230 (866723) 866723..867382 + 660 WP_000250274.1 hemolysin III family protein -
LCE25_RS04235 (867459) 867459..868439 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LCE25_RS04240 (868682) 868682..868948 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LCE25_RS04245 (868929) 868929..869336 + 408 WP_000244777.1 protein YgfX Toxin
LCE25_RS04250 (869376) 869376..869897 - 522 WP_001055874.1 flavodoxin FldB -
LCE25_RS04255 (870009) 870009..870905 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LCE25_RS04260 (870930) 870930..871640 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LCE25_RS04265 (871646) 871646..873379 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T217745 WP_000244777.1 NZ_CP083888:868929-869336 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T217745 NZ_CP114761:c5790683-5790405 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAAGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT217745 WP_000354046.1 NZ_CP083888:868682-868948 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT217745 NZ_CP114761:c5790393-5790088 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGAAGATCTCTCC
AACTGCTCTAGCACGCGCTTTGAACGTCTCCGCCCCGACAGTGAACGATATTGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGCCTGGGGCGTTACTTTGACACGTCCGCTCAGTTCTGGATGAATCTTCAGAGTGAGTATTCGTTGGCA
ACTGCTTATGCGGCGAACGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References