Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3422879..3423497 Replicon chromosome
Accession NZ_CP083886
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LCE23_RS17435 Protein ID WP_001291435.1
Coordinates 3423279..3423497 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LCE23_RS17430 Protein ID WP_000344800.1
Coordinates 3422879..3423253 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS17420 (3417968) 3417968..3419161 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LCE23_RS17425 (3419184) 3419184..3422333 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LCE23_RS17430 (3422879) 3422879..3423253 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LCE23_RS17435 (3423279) 3423279..3423497 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LCE23_RS17440 (3423669) 3423669..3424220 + 552 WP_000102536.1 maltose O-acetyltransferase -
LCE23_RS17445 (3424336) 3424336..3424806 + 471 WP_000136192.1 YlaC family protein -
LCE23_RS17450 (3424970) 3424970..3426520 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LCE23_RS17455 (3426562) 3426562..3426915 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LCE23_RS17465 (3427294) 3427294..3427605 + 312 WP_000409911.1 MGMT family protein -
LCE23_RS17470 (3427636) 3427636..3428208 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217738 WP_001291435.1 NZ_CP083886:3423279-3423497 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T217738 NZ_CP114761:c148131-147850 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT217738 WP_000344800.1 NZ_CP083886:3422879-3423253 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT217738 NZ_CP114761:c148355-148128 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References