Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 136189..136714 Replicon plasmid unnamed1
Accession NZ_CP083884
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LCE23_RS00755 Protein ID WP_001159871.1
Coordinates 136409..136714 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag LCE23_RS00750 Protein ID WP_000813630.1
Coordinates 136189..136407 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS00730 (133162) 133162..133578 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
LCE23_RS00735 (133575) 133575..133805 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin -
LCE23_RS00740 (134224) 134224..134913 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
LCE23_RS00745 (134944) 134944..135633 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
LCE23_RS00750 (136189) 136189..136407 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LCE23_RS00755 (136409) 136409..136714 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LCE23_RS00760 (136715) 136715..137461 + 747 WP_072748653.1 site-specific integrase -
LCE23_RS00765 (138161) 138161..138310 + 150 Protein_151 RepB family plasmid replication initiator protein -
LCE23_RS00775 (139716) 139716..140162 + 447 WP_000616196.1 hypothetical protein -
LCE23_RS00780 (140258) 140258..140515 - 258 WP_015387373.1 hypothetical protein -
LCE23_RS00785 (141244) 141244..141456 + 213 WP_001523378.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ant(3'')-Ia / dfrA1 / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD iucA / iucB / iucC / iucD / iutA 1..164740 164740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T217720 WP_001159871.1 NZ_CP083884:136409-136714 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T217720 NZ_CP114592:c4581024-4580713 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT217720 WP_000813630.1 NZ_CP083884:136189-136407 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT217720 NZ_CP114592:c4580712-4580422 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References