Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 133162..133805 Replicon plasmid unnamed1
Accession NZ_CP083884
Organism Escherichia coli strain NDM2

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag LCE23_RS00730 Protein ID WP_001044768.1
Coordinates 133162..133578 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag LCE23_RS00735 Protein ID WP_001261287.1
Coordinates 133575..133805 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE23_RS00710 (128192) 128192..129757 - 1566 WP_001128474.1 AAA family ATPase -
LCE23_RS00715 (129832) 129832..130248 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
LCE23_RS00720 (130245) 130245..130475 - 231 WP_088569139.1 type II toxin-antitoxin system VapB family antitoxin -
LCE23_RS00725 (130862) 130862..133000 + 2139 WP_001513523.1 AAA family ATPase -
LCE23_RS00730 (133162) 133162..133578 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
LCE23_RS00735 (133575) 133575..133805 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LCE23_RS00740 (134224) 134224..134913 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
LCE23_RS00745 (134944) 134944..135633 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
LCE23_RS00750 (136189) 136189..136407 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
LCE23_RS00755 (136409) 136409..136714 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
LCE23_RS00760 (136715) 136715..137461 + 747 WP_072748653.1 site-specific integrase -
LCE23_RS00765 (138161) 138161..138310 + 150 Protein_151 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ant(3'')-Ia / dfrA1 / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD iucA / iucB / iucC / iucD / iutA 1..164740 164740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T217719 WP_001044768.1 NZ_CP083884:c133578-133162 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T217719 NZ_CP114592:c4298973-4298800 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT217719 WP_001261287.1 NZ_CP083884:c133805-133575 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT217719 NZ_CP114592:c4299297-4299001 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References