Detailed information of TA system TA217718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 129832..130475
• Replicon: plasmid unnamed1
• Accession: NZ_CP083884
• Organism: Escherichia coli strain NDM2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: LCE23_RS00715
• Protein ID: WP_001034044.1
• Coordinates: 129832..130248 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: LCE23_RS00720
• Protein ID: WP_088569139.1
• Coordinates: 130245..130475 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE23_RS00700 (126234)	126234..126931	+	698	WP_223155668.1	IS1-like element IS1A family transposase	-
LCE23_RS00705 (127185)	127185..128207	-	1023	WP_000361402.1	helicase UvrD	-
LCE23_RS00710 (128192)	128192..129757	-	1566	WP_001128474.1	AAA family ATPase	-
LCE23_RS00715 (129832)	129832..130248	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCE23_RS00720 (130245)	130245..130475	-	231	WP_088569139.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LCE23_RS00725 (130862)	130862..133000	+	2139	WP_001513523.1	AAA family ATPase	-
LCE23_RS00730 (133162)	133162..133578	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	-
LCE23_RS00735 (133575)	133575..133805	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	-
LCE23_RS00740 (134224)	134224..134913	+	690	WP_001513524.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iucA / iucB / iucC / iucD / iutA	1..164740	164740
	-	flank	IS/Tn	-	-	126428..126931	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T217718 WP_001034044.1 NZ_CP083884:c130248-129832 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T217718 NZ_CP114592:c4202457-4202107 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8761.89 Da
• Isoelectric Point: 4.3895

>AT217718 WP_088569139.1 NZ_CP083884:c130475-130245 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKAEPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT217718 NZ_CP114592:c4202702-4202451 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A829CHW1