Detailed information of TA system TA217714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 32290..32915
• Replicon: plasmid unnamed1
• Accession: NZ_CP083884
• Organism: Escherichia coli strain NDM2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCE23_RS00215
• Protein ID: WP_000911313.1
• Coordinates: 32290..32688 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: LCE23_RS00220
• Protein ID: WP_000450520.1
• Coordinates: 32688..32915 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE23_RS00200 (28570)	28570..29067	+	498	WP_000605857.1	entry exclusion protein	-
LCE23_RS00205 (29099)	29099..29830	+	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
LCE23_RS00210 (30083)	30083..32281	+	2199	WP_001593043.1	type IV conjugative transfer system coupling protein TraD	-
LCE23_RS00215 (32290)	32290..32688	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
LCE23_RS00220 (32688)	32688..32915	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / floR / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iucA / iucB / iucC / iucD / iutA	1..164740	164740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T217714 WP_000911313.1 NZ_CP083884:c32688-32290 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T217714 NZ_CP114592:3519910-3520128 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT217714 WP_000450520.1 NZ_CP083884:c32915-32688 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT217714 NZ_CP114592:3519510-3519884 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2