Detailed information of TA system TA217710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 21024..21489
• Replicon: plasmid unnamed2
• Accession: NZ_CP083882
• Organism: Escherichia coli strain NDM3

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: LCE26_RS23970
• Protein ID: WP_001230707.1
• Coordinates: 21024..21275 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: LCE26_RS23975
• Protein ID: WP_001541977.1
• Coordinates: 21262..21489 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE26_RS23945 (LCE26_23945)	18293..18622	+	330	WP_001541974.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LCE26_RS23950 (LCE26_23950)	18603..18884	+	282	WP_000780222.1	helix-turn-helix transcriptional regulator	-
LCE26_RS23955 (LCE26_23955)	18899..19225	+	327	WP_001541975.1	hypothetical protein	-
LCE26_RS23960 (LCE26_23960)	19865..20380	+	516	WP_001025395.1	J domain-containing protein	-
LCE26_RS23965 (LCE26_23965)	20485..21027	+	543	WP_001083906.1	hypothetical protein	-
LCE26_RS23970 (LCE26_23970)	21024..21275	+	252	WP_001230707.1	hypothetical protein	Toxin
LCE26_RS23975 (LCE26_23975)	21262..21489	+	228	WP_001541977.1	hypothetical protein	Antitoxin
LCE26_RS23980 (LCE26_23980)	21943..22242	+	300	WP_001541978.1	hypothetical protein	-
LCE26_RS23985 (LCE26_23985)	22335..22685	+	351	WP_001541979.1	hypothetical protein	-
LCE26_RS23990 (LCE26_23990)	22959..23132	+	174	WP_071821486.1	type II toxin-antitoxin system HicB family antitoxin	-
LCE26_RS23995 (LCE26_23995)	23496..23999	+	504	WP_024190319.1	DNA distortion polypeptide 1	-
LCE26_RS24000 (LCE26_24000)	24003..25232	+	1230	WP_001541982.1	DNA relaxase/nickase TaxC	-
LCE26_RS24005 (LCE26_24005)	25309..25602	+	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LCE26_RS24010 (LCE26_24010)	25599..25814	+	216	WP_000760375.1	EexN family lipoprotein	-
LCE26_RS24015 (LCE26_24015)	25991..26167	+	177	WP_000660496.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50447	50447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T217710 WP_001230707.1 NZ_CP083882:21024-21275 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T217710 NZ_CP114592:c2458984-2458808 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.00 Da
• Isoelectric Point: 9.1450

>AT217710 WP_001541977.1 NZ_CP083882:21262-21489 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRQSK

Nucleotide

• Length: 228 bp

>AT217710 NZ_CP114592:c2458762-2458346 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: U9ZUY2