Detailed information of TA system TA021771

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4708245..4709079
• Replicon: chromosome
• Accession: NC_008563
• Organism: Escherichia coli APEC O1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: APECO1_RS23680
• Protein ID: WP_000854820.1
• Coordinates: 4708245..4708622 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: APECO1_RS23685
• Protein ID: WP_001285610.1
• Coordinates: 4708711..4709079 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO1_RS23655	4704808..4706346	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
APECO1_RS23665	4707181..4707741	-	561	Protein_4480	DUF4942 domain-containing protein	-
APECO1_RS31335	4707826..4708023	-	198	WP_085949158.1	DUF957 domain-containing protein	-
APECO1_RS31340	4708051..4708248	-	198	Protein_4482	hypothetical protein	-
APECO1_RS23680	4708245..4708622	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
APECO1_RS23685	4708711..4709079	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
APECO1_RS23690	4709159..4709380	-	222	WP_000692347.1	DUF987 domain-containing protein	-
APECO1_RS23695	4709467..4709943	-	477	WP_001186193.1	RadC family protein	-
APECO1_RS23700	4709958..4710437	-	480	WP_000706978.1	antirestriction protein	-
APECO1_RS31650	4710537..4710677	+	141	WP_000997937.1	hypothetical protein	-
APECO1_RS23710	4710703..4711521	-	819	WP_001175155.1	DUF945 domain-containing protein	-
APECO1_RS23715	4711611..4711844	-	234	WP_000883181.1	DUF905 family protein	-
APECO1_RS23720	4711850..4712527	-	678	WP_001097565.1	hypothetical protein	-
APECO1_RS23725	4712678..4713358	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T21771 WP_000854820.1 NC_008563:c4708622-4708245 [Escherichia coli APEC O1]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T21771 NC_008563:c4708622-4708245 [Escherichia coli APEC O1]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT21771 WP_001285610.1 NC_008563:c4709079-4708711 [Escherichia coli APEC O1]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT21771 NC_008563:c4709079-4708711 [Escherichia coli APEC O1]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6