Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 44275..44800 Replicon plasmid unnamed1
Accession NZ_CP083881
Organism Escherichia coli strain NDM3

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag LCE26_RS23135 Protein ID WP_001159868.1
Coordinates 44495..44800 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag LCE26_RS23130 Protein ID WP_000813634.1
Coordinates 44275..44493 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE26_RS23105 (39607) 39607..39834 - 228 WP_013023899.1 abortive infection family protein -
LCE26_RS23110 (39913) 39913..40610 + 698 WP_224434100.1 IS1-like element IS1B family transposase -
LCE26_RS23115 (40739) 40739..41436 + 698 WP_224434100.1 IS1-like element IS1B family transposase -
LCE26_RS23120 (41636) 41636..42568 - 933 WP_058682609.1 S-4TM family putative pore-forming effector -
LCE26_RS23125 (42572) 42572..43567 - 996 WP_000246636.1 hypothetical protein -
LCE26_RS23130 (44275) 44275..44493 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LCE26_RS23135 (44495) 44495..44800 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
LCE26_RS23140 (44801) 44801..45607 + 807 WP_000016982.1 site-specific integrase -
LCE26_RS23145 (46381) 46381..47136 + 756 WP_000852146.1 replication initiation protein RepE -
LCE26_RS23150 (47724) 47724..48890 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 - 1..159955 159955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T217706 WP_001159868.1 NZ_CP083881:44495-44800 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T217706 NZ_CP114592:1830920-1831171 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT217706 WP_000813634.1 NZ_CP083881:44275-44493 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT217706 NZ_CP114592:1831168-1831422 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References