Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 31171..31730 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP083881 | ||
| Organism | Escherichia coli strain NDM3 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | V0SVC8 |
| Locus tag | LCE26_RS23050 | Protein ID | WP_000421272.1 |
| Coordinates | 31455..31730 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | LCE26_RS23045 | Protein ID | WP_001387467.1 |
| Coordinates | 31171..31455 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCE26_RS23015 (26639) | 26639..26986 | - | 348 | WP_000679427.1 | quaternary ammonium compound efflux SMR transporter QacE delta 1 | - |
| LCE26_RS23020 (27103) | 27103..27941 | - | 839 | Protein_32 | AadA family aminoglycoside 3''-O-nucleotidyltransferase | - |
| LCE26_RS23025 (27947) | 27947..28237 | - | 291 | WP_001336345.1 | DUF1010 domain-containing protein | - |
| LCE26_RS23030 (28349) | 28349..28846 | - | 498 | WP_001083725.1 | trimethoprim-resistant dihydrofolate reductase DfrA12 | - |
| LCE26_RS23035 (28991) | 28991..29857 | + | 867 | Protein_35 | class 1 integron integrase IntI1 | - |
| LCE26_RS23040 (29912) | 29912..30616 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| LCE26_RS23045 (31171) | 31171..31455 | + | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| LCE26_RS23050 (31455) | 31455..31730 | + | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LCE26_RS23060 (32613) | 32613..32816 | + | 204 | Protein_40 | hypothetical protein | - |
| LCE26_RS23065 (32941) | 32941..33069 | - | 129 | Protein_41 | IS481 family transposase | - |
| LCE26_RS23070 (33102) | 33102..34271 | - | 1170 | WP_001072355.1 | hypothetical protein | - |
| LCE26_RS23075 (34803) | 34803..35071 | + | 269 | Protein_43 | type II toxin-antitoxin system ParD family antitoxin | - |
| LCE26_RS23080 (35058) | 35058..35347 | + | 290 | Protein_44 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LCE26_RS23085 (35414) | 35414..35596 | + | 183 | WP_042344623.1 | hypothetical protein | - |
| LCE26_RS23090 (35717) | 35717..36457 | + | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 | - | 1..159955 | 159955 | |
| - | inside | IScluster/Tn | tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 | - | 602..32349 | 31747 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10749.45 Da Isoelectric Point: 10.0871
>T217705 WP_000421272.1 NZ_CP083881:31455-31730 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T217705 NZ_CP114592:1307694-1308092 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT217705 WP_001387467.1 NZ_CP083881:31171-31455 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT217705 NZ_CP114592:1307467-1307694 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0SVC8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |