Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4151585..4152180 Replicon chromosome
Accession NZ_CP083880
Organism Escherichia coli strain NDM3

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag LCE26_RS20115 Protein ID WP_000239579.1
Coordinates 4151585..4151935 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag LCE26_RS20120 Protein ID WP_001223208.1
Coordinates 4151929..4152180 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE26_RS20095 (4147031) 4147031..4148053 - 1023 WP_001295197.1 ABC transporter permease -
LCE26_RS20100 (4148067) 4148067..4149569 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
LCE26_RS20105 (4149709) 4149709..4150665 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LCE26_RS20110 (4150975) 4150975..4151505 + 531 WP_000055072.1 inorganic diphosphatase -
LCE26_RS20115 (4151585) 4151585..4151935 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
LCE26_RS20120 (4151929) 4151929..4152180 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LCE26_RS20125 (4152392) 4152392..4152733 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
LCE26_RS20130 (4152736) 4152736..4156515 - 3780 WP_000060978.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T217703 WP_000239579.1 NZ_CP083880:c4151935-4151585 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T217703 NZ_CP114592:997441-997776 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT217703 WP_001223208.1 NZ_CP083880:c4152180-4151929 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT217703 NZ_CP114592:997193-997441 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References