Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4696995..4697492 Replicon chromosome
Accession NC_008563
Organism Escherichia coli APEC O1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag APECO1_RS23630 Protein ID WP_001173343.1
Coordinates 4697319..4697492 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag APECO1_RS23625 Protein ID WP_001312331.1
Coordinates 4696995..4697291 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO1_RS23605 4693725..4694444 - 720 WP_000611291.1 two-component system response regulator DcuR -
APECO1_RS23610 4694441..4696072 - 1632 WP_001216482.1 sensor histidine kinase -
APECO1_RS23615 4696253..4696483 + 231 WP_000373293.1 (4Fe-4S)-binding protein -
APECO1_RS23620 4696495..4696767 + 273 WP_000405647.1 N-acetyltransferase -
APECO1_RS23625 4696995..4697291 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
APECO1_RS23630 4697319..4697492 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
APECO1_RS23635 4697611..4699128 - 1518 WP_000003806.1 lysine--tRNA ligase -
APECO1_RS23640 4699365..4700822 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T21770 WP_001173343.1 NC_008563:4697319-4697492 [Escherichia coli APEC O1]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T21770 NC_008563:4697319-4697492 [Escherichia coli APEC O1]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT21770 WP_001312331.1 NC_008563:4696995-4697291 [Escherichia coli APEC O1]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT21770 NC_008563:4696995-4697291 [Escherichia coli APEC O1]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References