Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3443532..3444150 Replicon chromosome
Accession NZ_CP083880
Organism Escherichia coli strain NDM3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LCE26_RS16650 Protein ID WP_001291435.1
Coordinates 3443932..3444150 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LCE26_RS16645 Protein ID WP_000344800.1
Coordinates 3443532..3443906 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE26_RS16635 (3438621) 3438621..3439814 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LCE26_RS16640 (3439837) 3439837..3442986 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LCE26_RS16645 (3443532) 3443532..3443906 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LCE26_RS16650 (3443932) 3443932..3444150 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LCE26_RS16655 (3444322) 3444322..3444873 + 552 WP_000102564.1 maltose O-acetyltransferase -
LCE26_RS16660 (3444989) 3444989..3445459 + 471 WP_000136192.1 YlaC family protein -
LCE26_RS16665 (3445623) 3445623..3447173 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LCE26_RS16670 (3447215) 3447215..3447568 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LCE26_RS16680 (3447947) 3447947..3448258 + 312 WP_000409911.1 MGMT family protein -
LCE26_RS16685 (3448289) 3448289..3448861 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217699 WP_001291435.1 NZ_CP083880:3443932-3444150 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T217699 NZ_CP114591:454012-454311 [Salinivibrio kushneri]
ATGAAGCCATTTACGCTGACTATCGCCGCTAAAACGGACTTAAGGAATATCGCGTTGTTTACCCAACGTCGTTGGGGAAA
GGGACAGCGCAATCTATATGTAAAACGGTTCGATGACACCTTTTGGTTATTAGCTGAAAACCCAGACATGGGGAAACCAT
GTGATGACATCCGATCAGGATATCGTAAATTTCCTCTTGGCAGCCATATTGTTTTTTATCAACCCAAGGGGAATCATCAC
ATTAAAGTCGTTCGAATCCTGCATAAAAGTATGGACGTAAATCCAGCGTTGGGTATTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT217699 WP_000344800.1 NZ_CP083880:3443532-3443906 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT217699 NZ_CP114591:453773-454015 [Salinivibrio kushneri]
ATGGCAAAGAATACAAGTATTACCCTAGGTGAACACTTCGATGGCTTTATCGCTAGCCAAATCGAAACGGGGCGTTACAG
CTCGACAAGTGAAGTCGTTCGCGCCGCACTGCGTTTACTCGAAAACCAAGAGACAAAACTGCATACACTGCGTCAACTTC
TCGTAGAGGGCGAACAAAGTGGCGATGCTGAGTATGACATCGATAACTTTATCCAAGAAATCGATGCCGAAGATACGCAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References