Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 860327..860981 Replicon chromosome
Accession NZ_CP083880
Organism Escherichia coli strain NDM3

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LCE26_RS04215 Protein ID WP_000244781.1
Coordinates 860574..860981 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LCE26_RS04210 Protein ID WP_000354046.1
Coordinates 860327..860593 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE26_RS04190 (856415) 856415..857848 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
LCE26_RS04195 (857893) 857893..858204 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
LCE26_RS04200 (858368) 858368..859027 + 660 WP_000250269.1 hemolysin III family protein -
LCE26_RS04205 (859104) 859104..860084 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
LCE26_RS04210 (860327) 860327..860593 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LCE26_RS04215 (860574) 860574..860981 + 408 WP_000244781.1 protein YgfX Toxin
LCE26_RS04220 (861021) 861021..861542 - 522 WP_001521233.1 flavodoxin FldB -
LCE26_RS04225 (861654) 861654..862550 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LCE26_RS04230 (862575) 862575..863285 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LCE26_RS04235 (863291) 863291..865024 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T217686 WP_000244781.1 NZ_CP083880:860574-860981 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T217686 NZ_CP114584:1190367-1190627 [Salinivibrio proteolyticus]
ATGAAAATCATTTCGTTTACTGAAGCGAGAAATGGCCTCAAAACGGTATTAGATGGGGTGGTTGATGACGCTGATACCAC
TATCATCACCCGACGTGATGCGGAAGACGCGGTGGTGATGTCGCTTGATTACTACAACAGCCTTATGGAAACCGTTCATC
TGTTGCGCTCACCCGCCAATGCTGAGCACCTAAATCGGTCTATTACTCAGTACAAGATGGGGAAAGCGATGGAGCACGCG
TTAGTCGATAGCGATGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT217686 WP_000354046.1 NZ_CP083880:860327-860593 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT217686 NZ_CP114584:1190620-1190883 [Salinivibrio proteolyticus]
ATGAGTAGTCGCTTACTTTGCTGGACGGATGATGCGTGGCGTGACTACGTGTACTGGCAAAGCCAGGATAGAAAAACACT
CAAGCGAATCAACAAGCTCATCCTCGATGTAAAACGAACGCCATTCGAGGGCATAGGTAAGCCCGAAGCACTAAAAGAAA
ACCTGACCGGCTTCTGGTCGCGGCGTATTGATGATACCAATCGGCTGGTTTACGCCGTGGATGATACGTCTATTACGATC
CTATCTTGCCGATATCACTATTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PAM6


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References