217681

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 119493..120052 Replicon plasmid unnamed1
Accession NZ_CP083878
Organism Escherichia coli strain NDM4

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag LCE22_RS23575 Protein ID WP_000421272.1
Coordinates 119777..120052 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag LCE22_RS23570 Protein ID WP_001387467.1
Coordinates 119493..119777 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE22_RS23540 (114961) 114961..115308 - 348 WP_000679427.1 quaternary ammonium compound efflux SMR transporter QacE delta 1 -
LCE22_RS23545 (115425) 115425..116263 - 839 Protein_138 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
LCE22_RS23550 (116269) 116269..116559 - 291 WP_001336345.1 DUF1010 domain-containing protein -
LCE22_RS23555 (116671) 116671..117168 - 498 WP_001083725.1 trimethoprim-resistant dihydrofolate reductase DfrA12 -
LCE22_RS23560 (117313) 117313..118179 + 867 Protein_141 class 1 integron integrase IntI1 -
LCE22_RS23565 (118234) 118234..118938 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LCE22_RS23570 (119493) 119493..119777 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
LCE22_RS23575 (119777) 119777..120052 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCE22_RS23585 (120935) 120935..121138 + 204 Protein_146 hypothetical protein -
LCE22_RS23590 (121263) 121263..121391 - 129 Protein_147 IS481 family transposase -
LCE22_RS23595 (121424) 121424..122593 - 1170 WP_001072355.1 hypothetical protein -
LCE22_RS23600 (123125) 123125..123393 + 269 Protein_149 type II toxin-antitoxin system ParD family antitoxin -
LCE22_RS23605 (123380) 123380..123669 + 290 Protein_150 type II toxin-antitoxin system RelE/ParE family toxin -
LCE22_RS23610 (123736) 123736..123918 + 183 WP_042344623.1 hypothetical protein -
LCE22_RS23615 (124039) 124039..124779 + 741 WP_001066953.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 - 1..158041 158041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T217681 WP_000421272.1 NZ_CP083878:119777-120052 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T217681 NZ_CP114576:c71994-70690 [Legionella pneumophila]
ATGGTAATGCGTAAATTATACATATTGATGAACAATGAGCTTGTGGGGGAGCTTACCCAGGATAATGCAGGAGGCTTGAG
TTTTTCCTACGTTGCTGACTGGATTTCCAAGCCACATTCACGCCCAGTTTCTTTATCGTTACCGTTAGTAAATCAAAGGT
TTTCAGGAGAAGTTGTTTATAACTTTTTTGATAATTTACTTCCCGATAATCCACAGATAAGAGCGAGAATTCAAGCTCGT
TTTCAGGCAAAATCATCGCGACCGTTTGATTTATTGGCGTTAATTGGCGGTGATTGTGTTGGTGCAATACAAATTGTTGA
ACGTATTCCTCAGACACAGCGTCTAAGCATTGAAAGTCACGTTGTCAGTGAGGCCGAGATAGCTCGTACTTTACGTTCTT
ACCAAACCTCACCACTGGGAATGGATAAAGAGGATGATGAGTTTAGAATCTCAATAGCCGGTGCTCAGGAAAAAACAGCT
TTTCTATATTACCAAAATGAATGGCATAAACCATTTGGACCTACACCAACAACTCACATATTCAAATTACCTATTGGATA
TTTACCACATCAAGGAATTGATTTAAGTGAGAGTTGTGAAAATGAGCATCTCTGTTTACTACTAGCCAGCAAATTTGGTT
TAGAGGTTGCAAAATCAAAAATCTTACAATTCGAAGATGAAAAGGTGCTGGTTGTCGAAAGATTTGATAGAAAATGGAGT
CGAGATAATCGTTATATACTCAGGTTACCGCAAGAAGACCTATGCCAAGCGTTGGGTTATTCACCAAATTTAAAATATCA
GTCAGATGGTGGCCCCGGTATTAAAGAAATTATGCAGCTACTTAATGGCTCACAGCAGCCTCAAAAAGATAGAGATGAAT
TTTTCAGAGCACAAGTTGTTTACTGGTGCCTTGGTGCTATTGATGCGCATGCCAAAAACTTCAGTATAAGTATACAACCA
CAAGGCGGTTACCAGCTCACCCCCTTATACGACATCATTTCAGCCTATCCTATCGTGGAACAAAAACAGCTATCCTTTAA
GAAATTGAAAATGGCAATGGCTTTATATGGTAAAAATAGTCATTACAAGATTGACGGTATTCAAAAAAGACACTTTATTG
AAACAGCAAAATACGTTCAATATTCTAAAATTAAAGCAGCTCAAATTTTTGAACAAGTGATTGACCGTATTGATTCTGCT
ATCGATGAGGTCTCAACACAATTACCAAACCACTTCCCAGAAAAGATTGCAGATCCAATCTTCAAAGGGATGAGAGCGTT
TAAGGAGCGAATGATATCCACATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT217681 WP_001387467.1 NZ_CP083878:119493-119777 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT217681 NZ_CP114576:c72227-71988 [Legionella pneumophila]
ATGATCATTAGCTCAGCAAAAGATTTAGCATTATATTTAAATGACAAGCGCAAACAACTCAAAATGAGTCAATCTGAAGT
TGCCGATTTAGTCGGCTTAAAACAGGATACCATATCAAAGTTTGAAAATAGCCCTGATAATAGTCGAATTGATACCTTAT
TTAGAATATTGTCCGCCCTAAATTTAAATATGAGCCTTGTTGAAAAAGATCAAAAAACTCATGAAGAGATAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References