Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4253871..4254368 Replicon chromosome
Accession NZ_CP083877
Organism Escherichia coli strain NDM4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LCE22_RS20645 Protein ID WP_001173343.1
Coordinates 4253871..4254044 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LCE22_RS20650 Protein ID WP_000398619.1
Coordinates 4254072..4254368 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE22_RS20635 (4250541) 4250541..4251998 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LCE22_RS20640 (4252235) 4252235..4253752 + 1518 WP_001295074.1 lysine--tRNA ligase -
LCE22_RS20645 (4253871) 4253871..4254044 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LCE22_RS20650 (4254072) 4254072..4254368 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCE22_RS20655 (4254595) 4254595..4254867 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LCE22_RS20660 (4254879) 4254879..4255109 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LCE22_RS20665 (4255290) 4255290..4256921 + 1632 WP_001216477.1 sensor histidine kinase -
LCE22_RS20670 (4256918) 4256918..4257637 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T217677 WP_001173343.1 NZ_CP083877:c4254044-4253871 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T217677 NZ_CP114571:c4786622-4786251 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT217677 WP_000398619.1 NZ_CP083877:c4254368-4254072 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT217677 NZ_CP114571:c4786867-4786625 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References