Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 159263..159822 Replicon plasmid unnamed3
Accession NZ_CP083875
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag LCE27_RS24145 Protein ID WP_000421272.1
Coordinates 159263..159538 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag LCE27_RS24150 Protein ID WP_001387467.1
Coordinates 159538..159822 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS24105 (154536) 154536..155276 - 741 Protein_180 site-specific integrase -
LCE27_RS24110 (155397) 155397..155579 - 183 WP_042344623.1 hypothetical protein -
LCE27_RS24115 (155646) 155646..155935 - 290 Protein_182 type II toxin-antitoxin system RelE/ParE family toxin -
LCE27_RS24120 (155922) 155922..156191 - 270 WP_224450645.1 type II toxin-antitoxin system ParD family antitoxin -
LCE27_RS24125 (156723) 156723..157892 + 1170 WP_001072355.1 hypothetical protein -
LCE27_RS24130 (157925) 157925..158053 + 129 Protein_185 IS481 family transposase -
LCE27_RS24135 (158178) 158178..158381 - 204 Protein_186 hypothetical protein -
LCE27_RS24140 (158451) 158451..159147 + 697 Protein_187 IS1 family transposase -
LCE27_RS24145 (159263) 159263..159538 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LCE27_RS24150 (159538) 159538..159822 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
LCE27_RS24155 (160376) 160376..161080 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
LCE27_RS24160 (161135) 161135..162001 - 867 Protein_191 class 1 integron integrase IntI1 -
LCE27_RS24165 (162146) 162146..162643 + 498 WP_001083725.1 trimethoprim-resistant dihydrofolate reductase DfrA12 -
LCE27_RS24170 (162755) 162755..163045 + 291 WP_001336345.1 DUF1010 domain-containing protein -
LCE27_RS24175 (163051) 163051..163889 + 839 Protein_194 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
LCE27_RS24180 (164006) 164006..164353 + 348 WP_000679427.1 quaternary ammonium compound efflux SMR transporter QacE delta 1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-5 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / dfrA12 / aadA2 / qacE / sul1 - 1..170625 170625
- inside IScluster/Tn dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 - 158646..170313 11667


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T217655 WP_000421272.1 NZ_CP083875:c159538-159263 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T217655 NZ_CP114551:c1012031-1011756 [Xylella fastidiosa subsp. pauca]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTGGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGACGAGAATGCTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT217655 WP_001387467.1 NZ_CP083875:c159822-159538 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT217655 NZ_CP114551:c1012242-1012015 [Xylella fastidiosa subsp. pauca]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTGAACTATCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References